Rediscovery of the nucleolinus, a dynamic RNA-rich organelle associated with the nucleolus, spindle, and centrosomes.

Proceedings of the National Academy of Sciences of the United States of America
Mary Anne AlliegroMark C Alliegro

Abstract

The nucleolinus is an RNA-rich compartment, closely apposed to or embedded within the nucleolus. Discovered over 150 y ago, fewer than two dozen articles have been published on the nucleolinus, probably because complex histochemical stains are required for its visualization in the great majority of cells. The nucleolinus has been reported in invertebrate oocytes, mammalian and amphibian epithelial cells, neurons, and several transformed cell lines. A prominent nucleolinus, clearly visible with transmitted light microscopes at 10x magnification, is present in each oocyte of the surf clam, Spisula solidissima. We observed a consistent relationship between the nucleolinus and the developing meiotic apparatus following Spisula oocyte activation. Through sonication and sucrose gradient fractionation of purified oocyte nuclei, we isolated nucleolini, extracted their RNA, and prepared an in situ riboprobe (NLi-1), which is associated specifically with the nucleolinus, confirming its unique composition. Other in situ observations revealed a NLi-1 and nucleolinar association with the developing spindle and centrosomes. Laser microsurgery that targeted the nucleolinus resulted in failed meiotic cell division in parthenogenetically activa...Continue Reading

References

Apr 10, 1992·Science·R E PalazzoC L Rieder
Nov 1, 1980·Experimental Cell Research·G G Maul, N Avdalović
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Wu, R E Palazzo
Sep 5, 1958·Annals of the New York Academy of Sciences·M J KOPAC, G M MATEYKO
May 1, 1959·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R LOVE, R H LILES
Jun 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·M E Gaulden, R P Perry
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mark C AlliegroRobert E Palazzo
May 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Mark C Alliegro, Mary Anne Alliegro
May 1, 1963·The Journal of Cell Biology·R Love, P Wildy

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Citations

Feb 3, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Mark C Alliegro
Feb 23, 2012·Journal of Biomedical Optics·Charlie ChandsawangbhuwanaMichael W Berns
Jun 10, 2011·Communicative & Integrative Biology·Mark C Alliegro
Jul 26, 2011·Biochimie·Malgorzata KlocSriyutha A Reddy
Mar 23, 2019·Frontiers in Genetics·Sarah C Hughes, Andrew J Simmonds

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