Redistribution and increase in cortical inositol 1,4,5-trisphosphate receptors after meiotic maturation of the mouse oocyte

Developmental Biology
Lisa M MehlmannD Kline

Abstract

Mouse oocytes develop sensitivity to inositol 1,4,5-trisphosphate (IP3) during oocyte maturation. We recently reported that a change in the organization of the endoplasmic reticulum (ER) during oocyte maturation may contribute to this enhanced sensitivity (Mehlmann et al., 1995, Dev. Biol. 170, 607-615). Here, we investigated whether there is an increase in the number of available IP3 receptors after maturation and whether there is a redistribution of IP3 receptors similar to the redistribution of the ER that occurs during maturation. Western blot analysis of the IP3 receptor in oocytes and eggs demonstrated a 1.8-fold increase in immunoreactive mass of the IP3 receptor following oocyte maturation. Microinjection of the function-blocking monoclonal antibody 18A10 inhibited IP3-induced Ca2+ release in a concentration-dependent manner in both eggs and oocytes. More antibody was required to inhibit Ca2+ release to the same extent in eggs compared to oocytes when both were injected with the same concentration of IP3, suggesting that eggs contain a greater number of functional IP3 receptors. Immunolocalization of the IP3 receptor revealed that receptors were present in large clusters, 1-2 microm in diameter, in the cortex of the mat...Continue Reading

Citations

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