Redistribution of G(q/11)alpha in the pituitary gonadotrope in response to a gonadotropin-releasing hormone agonist

Endocrinology
A CorneaP M Conn

Abstract

In the present study, we took advantage of high-resolution multilaser confocal microscopy to examine the distribution of the alpha-subunit of the guanyl nucleotide binding protein subfamily G(q/11) (G(q/11)alpha). Dispersed cultures of pituitary cells were prepared from female weanling rats, fixed, permeabilized, and then stained with monoclonal antiserum (mouse) to the gonadotrope-specific form of secretogranin (SIIp), which was then tagged with Texas Red. Accordingly, the subpopulation of gonadotropes (approximately 15% of total cells) could be identified against a background of other pituitary cell types. G(q/11)alpha was localized with antiserum made in rabbit, then tagged with fluorescein. Hoechst 33258 nuclear stain was also used in some experiments for topological reference. The data indicate localization of the G(q/11)alpha in a cellular region near the plasma membrane and external to the border of the layer occupied by secretory granules. In the absence of activation, there were an average of six clusters of G(q/11)alpha in a section 1 microm thick and through the center of the cell. This corresponds to an average of 60 clusters per cell, assuming a mean gonadotrope diameter of 10 microm. Following continuous treatment...Continue Reading

References

Jan 1, 1992·DNA and Cell Biology·W C ProbstS C Sealfon
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Aug 1, 1994·Journal of Neuroendocrinology·J C PainsonR Collu
Jun 1, 1997·Molecular Endocrinology·D StanislausP M Conn

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Citations

Sep 28, 2004·Theoretical Biology & Medical Modelling·Talitha M WashingtonP Michael Conn
Apr 28, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D TranG Guillon
May 20, 2000·The Journal of Biological Chemistry·C A Chen, D R Manning

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