Redistribution of Gαs in mouse salivary glands following β-adrenergic stimulation

Archives of Oral Biology
Arthur R HandRachael P Norris

Abstract

Signalling via β-adrenergic receptors activates heterotrimeric G-proteins, which dissociate into α and βγ subunits. In salivary glands, the α subunit of Gs stimulates adenylate cyclase, increasing cyclic AMP levels and promoting exocytosis. The goals of this study were to determine Gαs localization in salivary glands and whether it undergoes redistribution upon activation. Mouse parotid and submandibular (SMG) glands were fixed with paraformaldehyde and prepared for immunofluorescence labelling with anti-Gαs. In unstimulated parotid and SMG acinar cells, Gαs was localized mainly to basolateral membranes. Some parotid acinar cells also exhibited cytoplasmic fluorescence. Isoproterenol (IPR) stimulation resulted in decreased membrane fluorescence and increased cytoplasmic fluorescence, which appeared relatively uniform by 30 min. Beginning about 2 h after IPR, cytoplasmic fluorescence decreased and membrane fluorescence increased, approaching unstimulated levels in SMG acini by 4 h. Some parotid acini exhibited cytoplasmic fluorescence up to 8 h after IPR. The IPR-induced redistribution of Gαs was prevented (SMG) or reduced (parotid) by prior injection of propranolol. Striated duct cells of unstimulated mice exhibited general cyt...Continue Reading

References

Jul 15, 1992·The Biochemical Journal·E L WatsonK T Izutsu
Jul 1, 1994·Cell·P B Wedegaertner, H R Bourne
Aug 1, 1996·Molecular Biology of the Cell·P B WedegaertnerM von Zastrow
Jun 11, 1999·The Journal of Biological Chemistry·X LuoS Muallem
Feb 3, 1965·Biochemical and Biophysical Research Communications·A BDOLAH, M SCHRAMM
Sep 27, 2005·Physiological Reviews·Nina Wettschureck, Stefan Offermanns
Oct 26, 2005·The Journal of Cell Biology·Leon FreudzonLaurinda A Jaffe
Jun 15, 2007·Biochemistry·Yannick MarrariPhilip B Wedegaertner
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Apr 12, 2008·Biochimica Et Biophysica Acta·Oliver VöglerPablo V Escribá
Dec 23, 2009·The Journal of Physiology·Marcelo A CatalánJames E Melvin
Sep 13, 2011·Cellular Signalling·Thamara Hewavitharana, Philip B Wedegaertner

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