Redox- and calmodulin-dependent S-nitrosylation of the KCNQ1 channel

The Journal of Biological Chemistry
Ken AsadaTetsushi Furukawa

Abstract

Nitric oxide (NO) is a gaseous signal mediator showing numerous important biological effects. NO has been shown in many instances to exhibit its action via the protein S-nitrosylation mechanism, in which binding of NO to Cys residues regulate protein function independently of activation of soluble guanylate cyclase. The direct link between protein S-nitrosylation and functional modulation, however, has been demonstrated only in limited examples. Furthermore, although most proteins have more than one Cys residue, the mechanism by which a certain Cys becomes a specific target residue of S-nitrosylation is poorly understood. We have previously reported that NO regulates currents through the cardiac slowly activating delayed rectifier potassium channel (I(Ks)) irrespective of soluble guanylate cyclase activation. Here we demonstrate using a biotin-switch assay that NO induced S-nitrosylation of the alpha-subunit of the I(Ks) channel, KCNQ1, at Cys(445) in the C terminus. A redox motif flanking Cys(445) and the interaction of KCNQ1 with calmodulin are required for preferential S-nitrosylation of Cys(445). A patch clamp experiment shows that S-nitrosylation of Cys(445) modulates the KCNQ1/KCNE1 channel function. Our data provide a mo...Continue Reading

References

May 1, 1997·Neuron·J S StamlerN J Sucher
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·A NedospasovE Nudler
Feb 15, 2001·Nature Cell Biology·S R JaffreyS H Snyder
Feb 15, 2001·Nature Cell Biology·D T HessJ S Stamler
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·J SunG Meissner
Aug 17, 2002·Trends in Neurosciences·Stuart A LiptonLaurie A Bankston
Jan 24, 2003·Annual Review of Pharmacology and Toxicology·Colleen E ClancyRobert S Kass
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Douglas T HessJonathan S Stamler
May 20, 2005·Molecular Biology of the Cell·Nikita GamperMark S Shapiro
Sep 15, 2005·Circulation·Chang-Xi BaiTetsushi Furukawa
Jan 13, 2006·Nature Chemical Biology·Douglas A Mitchell, Michael A Marletta
Jan 1, 2008·The Journal of Biological Chemistry·Reuven WienerJoel A Hirsch
Jul 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kazuo UedaJonathan C Makielski

❮ Previous
Next ❯

Citations

Mar 1, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Puneet Anand, Jonathan S Stamler
Oct 4, 2012·The Journal of Biological Chemistry·Alexander W LohmanBrant E Isakson
Jul 10, 2012·Antioxidants & Redox Signaling·Bradley A MaronJoseph Loscalzo
Jul 1, 2010·Cardiovascular Research·Juan TamargoEva Delpón
Apr 26, 2013·Cardiovascular Research·Jordi Heijman, Dobromir Dobrev
Aug 19, 2009·Journal of Cardiovascular Pharmacology·Daniel R GonzalezJoshua M Hare
Aug 19, 2010·The Plant Cell·Christian LindermayrJörg Durner
Mar 6, 2010·Circulation Research·Brian LimaJonathan S Stamler
Dec 1, 2009·Respiratory Research·Amy N SavitskiJames L Kreindler
Jan 11, 2013·Biological & Pharmaceutical Bulletin·Junko Kurokawa, Tetsushi Furukawa
Mar 17, 2010·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Junko KurokawaTetsushi Furukawa
Feb 4, 2010·PLoS Computational Biology·Pei-Chi YangColleen E Clancy
Nov 26, 2013·Biochemistry·David BalchinHeini W Dirr
Oct 3, 2013·Advances in Therapy·Guoliang LiChaofeng Sun
May 4, 2011·Biochimica Et Biophysica Acta·Ivonne Hernandez Schulman, Joshua M Hare
Sep 4, 2009·Trends in Molecular Medicine·Matthew W FosterJonathan S Stamler
Aug 18, 2009·Journal of Molecular and Cellular Cardiology·Flavien CharpentierIsabelle Baró
Jun 21, 2013·Transfusion·Viachaslau BarodkaJoseph M Rifkind
Nov 28, 2012·Journal of Molecular Biology·Qiang XuDaniel L Minor
Dec 3, 2011·Brain Research·Alex H VielmaOliver Schmachtenberg
Dec 17, 2014·Biochimica Et Biophysica Acta·Carmen WolkeUwe Lendeckel
May 31, 2013·Neuron·Tomohiro NakamuraStuart A Lipton
Sep 13, 2011·Journal of Molecular and Cellular Cardiology·Elizabeth MurphyCharles Steenbergen
Apr 8, 2014·Antioxidants & Redox Signaling·Andrea Olschewski, Edward Kenneth Weir
Dec 30, 2014·Antioxidants & Redox Signaling·Florian VeitNorbert Weissmann
Sep 7, 2016·Pharmacology & Therapeutics·Junko KurokawaTetsushi Furukawa
Dec 21, 2018·Antioxidants & Redox Signaling·Laura Weise-CrossNikki L Jernigan
Jul 15, 2009·Journal of Cardiovascular Pharmacology·Junko Kurokawa, Hugues Abriel
Feb 19, 2010·Journal of Cardiovascular Pharmacology·Pei-Chi Yang, Colleen E Clancy
Aug 13, 2011·Animal Cells and Systems·Eun Jeoung LeeSang Sun Kang
Mar 26, 2011·Pharmacological Reviews·Irmgard TegederGerd Geisslinger
Apr 30, 2011·Journal of Cardiovascular Pharmacology and Therapeutics·John C SombergJanos Molnar
Mar 29, 2014·Experimental Biology and Medicine·Claudia PennaPasquale Pagliaro
Jan 2, 2021·Frontiers in Physiology·Hannah E BoycottPaul Robinson
Jan 23, 2021·Angiology·Teresa InfanteClaudio Napoli
Jan 13, 2021·International Journal of Molecular Sciences·Gizem Kayki-Mutlu, Walter J Koch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Cardiac Electrophysiology

Cardiac electrophysiology is the study of electrical activities of the heart and includes the assessment, diagnosis, and treatment of cardiac events. Find the latest research on cardiac electrophysiology here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.