PMID: 8954156Nov 15, 1996Paper

Redox control of RNA synthesis in potato mitochondria

European Journal of Biochemistry
S B WilsonC K Pearson

Abstract

This study shows that the incorporation of radiolabelled UTP into RNA in Percoll-gadient-purified potato mitochondria is regulated by the redox state of the mitochondrial electron transport chain. An early indication that there might be a redox effect on RNA synthesis was a decrease in UTP incorporation in incubates containing an oxidisable substrate, such as succinate or malate. Subsequent use of a variety of electron transport inhibitors acting at different points in the electron transport chain established that the redox state of the Rieske iron-sulphur protein was the major determinant of UTP incorporation. Inhibitors acting on the substrate side of the Rieske iron-sulphur protein, and causing oxidation of components on the oxygen side of their site of action, increased UTP incorporation into RNA. These included antimycin A, myxothiazole, and undecylhydroxydioxobenzothiazole at 500 nM. Inhibitors acting on the oxygen side of the Rieske iron-sulphur protein, and causing a reduction of components on the substrate side of the block, decreased UTP incorporation. These inhibitors were undecylhydroxydioxobenzothiazole at 25 nM and KCN. When phenazine methosulphate was present as an auto-oxidisable electron sink the effect of KCN ...Continue Reading

References

Nov 1, 1978·Archives of Biochemistry and Biophysics·H RobertsK Folkers
Jan 1, 1978·Methods in Enzymology·G S Wilson
Jan 22, 1992·Biochimica Et Biophysica Acta·J F Allen
Aug 15, 1989·The Biochemical Journal·C MacKintosh, P Cohen
Jan 1, 1986·Methods in Enzymology·G von Jagow, T A Link
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
Nov 1, 1973·Journal of Medicinal Chemistry·M D FriedmanK Folkers
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·J M EscoubasP G Falkowski
May 1, 1996·Biochemical Society Transactions·S B Wilson, C K Pearson
Feb 1, 1995·Redox Report : Communications in Free Radical Research·C A AllenJ F Allen

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Citations

Mar 26, 1999·European Journal of Biochemistry·T V VotyakovaS B Wilson
Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John F Allen
Aug 20, 2015·Proceedings of the National Academy of Sciences of the United States of America·John F Allen
Sep 26, 2013·Biochemical Society Transactions·John F Allen, Wilson B M de Paula
Jul 17, 2008·Comparative and Functional Genomics·John F Allen

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