Redox-linked protein dynamics of cytochrome c probed by time-resolved surface enhanced infrared absorption spectroscopy

Physical Chemistry Chemical Physics : PCCP
Nattawadee WisitruangsakulPeter Hildebrandt

Abstract

Time-resolved surface enhanced infrared absorption (SEIRA) spectroscopy is employed to analyse the dynamics of the protein structural changes coupled to the electron transfer process of immobilised cytochrome c (Cyt-c). Upon electrostatic binding of Cyt-c to Au electrodes coated with self-assembled monolayers (SAMs) of carboxyl-terminated thiols, cyclic voltammetric measurements demonstrate a reversible redox process with a redox potential that is similar to that of Cyt-c in solution, and a non-exponential distance-dependence of the electron transfer rate as observed previously (D. H. Murgida and P. Hildebrandt, Chem. Soc. Rev. 2008, 37, 937). On the basis of characteristic redox-state-sensitive amide I bands, the protein structural changes triggered by the electron transfer are monitored by rapid scan and step scan SEIRA spectroscopy in combination with the potential jump technique. Whereas the temporal evolution of the conjugate bands at 1693 and 1673 cm(-1) displays the same rate constants as electron transfer, the time-dependent changes of the 1660-cm(-1) band are slower by about a factor of 2. The study demonstrates that time-resolved SEIRA spectroscopy provides further information about the dynamics and mechanism of inter...Continue Reading

References

Feb 20, 1992·Journal of Molecular Biology·A M Berghuis, G D Brayer
Aug 30, 1983·Biochemical and Biophysical Research Communications·H Susi, D M Byler
Jul 18, 2001·Journal of the American Chemical Society·D H Murgida, P Hildebrandt
Aug 9, 2002·Journal of the American Chemical Society·Jianjun WeiDavid H Waldeck
Apr 14, 2005·Chemical Reviews·J Christopher LoveGeorge M Whitesides
Dec 17, 2005·Physical Chemistry Chemical Physics : PCCP·Daniel H Murgida, Peter Hildebrandt
Jan 24, 2007·Analytical and Bioanalytical Chemistry·Kenichi Ataka, Joachim Heberle
Feb 27, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Matheus T de GrootMarc T M Koper
Aug 7, 2007·Analytical Chemistry·Pablo RamírezJuan José Calvente
Apr 30, 2008·Chemical Society Reviews·Daniel H Murgida, Peter Hildebrandt

❮ Previous
Next ❯

Citations

Jul 7, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Kylie A Vincent
Oct 7, 2010·Biophysical Journal·Ekaterina ZaitsevaReiner Vogel
Feb 12, 2014·Metallomics : Integrated Biometal Science·Antonio RanieriMarco Sola
Feb 24, 2015·Chembiochem : a European Journal of Chemical Biology·Jan VöllerNediljko Budisa
Jun 15, 2010·Physical Chemistry Chemical Physics : PCCP·Murat SezerInez M Weidinger
Nov 15, 2011·Chemical Communications : Chem Comm·Gal SchkolnikPeter Hildebrandt
Apr 25, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Patrycja KielbInez M Weidinger
Aug 20, 2009·Physical Chemistry Chemical Physics : PCCP·P ZuoP Hildebrandt
Mar 24, 2020·Chemical Reviews·Victor A Lorenz-Fonfria
Nov 4, 2008·The Journal of Physical Chemistry. B·Jiu-Ju FengInez M Weidinger
Aug 16, 2018·The Journal of Physical Chemistry. B·Hernan BiavaPeter Hildebrandt
Mar 28, 2012·Journal of the American Chemical Society·Christophe RenaultBenoît Limoges
Jan 20, 2017·The Journal of Physical Chemistry. B·Tilman KottkeJoachim Heberle
Oct 14, 2017·Chemical Reviews·Damián Alvarez-PaggiDaniel H Murgida

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.