PMID: 6319123Dec 15, 1983Paper

Redox-linked proton translocation in the b-c1 complex from beef-heart mitochondria reconstituted into phospholipid vesicles. General characteristics and control of electron flow by delta micro H+

European Journal of Biochemistry
S PapaE Bellomo

Abstract

A study is presented of the characteristics of redox-linked proton translocation in the b-c1 complex isolated from beef-heart mitochondria and reconstituted into phospholipid vesicles. Measurements of the H+/e- stoichiometry, with three different methods, show that four protons are released from the vesicles per 2e- flowing from quinols to cytochrome c, two of these protons formally deriving from scalar oxidation of quinols by cytochrome c. This H+/e- stoicheiometry is independent of the initial redox state of the b-c1 complex (fully reduced or oxidized) and the rate of electron flow through the complex. It does not change in the pH range 6.0 - 7.2, but declines to 1.5 going with pH from 7.2 - 8.3. This decrease is accompanied by enhancement of the rate of electron flow in the coupled state. Collapse of delta psi effected by valinomycin addition to turning-over b-c1 vesicles resulted in substantial oxidation of cytochrome b-566 and comparable reduction of cytochrome c1, with little oxidation of cytochrome b-562. Nigericin alone had no effect on the steady-state redox levels of b and c cytochromes. Its addition in the presence of valinomycin caused oxidation of b cytochromes but no change in the redox state of cytochrome c1. Val...Continue Reading

References

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Citations

Feb 1, 1986·Journal of Bioenergetics and Biomembranes·D S Beattie
Apr 1, 1985·Journal of Bioenergetics and Biomembranes·M Degli Esposti, G Lenaz
Jun 1, 1993·Journal of Bioenergetics and Biomembranes·D S Beattie
Feb 1, 1995·Journal of Bioenergetics and Biomembranes·M LorussoS Papa
Jun 12, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Nicholas E KaragasLaura M Hunsicker-Wang
Dec 1, 1994·Journal of Bioenergetics and Biomembranes·S PapaN Capitanio
May 1, 1987·Biological Reviews of the Cambridge Philosophical Society·M D Brand, M P Murphy
Apr 23, 1991·European Journal of Biochemistry·M LorussoS Papa

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