PMID: 9183012May 1, 1997Paper

Redox-mediated regulation of p21(waf1/cip1) expression involves a post-transcriptional mechanism and activation of the mitogen-activated protein kinase pathway

European Journal of Biochemistry
F EspositoT Russo

Abstract

p21(waf1/cip1) gene expression is induced by DNA damage in cells with wild-type p53 and contributes to the arrest of cell growth. It was demonstrated that under many experimental conditions, including oxidative stress, p21(waf1/cip1) expression can be induced through p53-independent pathways. Since most of these experimental conditions induce the phosphorylation of mitogen-activated protein kinase (MAPK) and thus its activation, we evaluated p21(waf1/cip1) mRNA levels in cells exposed to an oxidative stress, induced by diethylmaleate (Et2Mal), and in which the MAPK pathway was blocked. The expression of a dominant-negative mutant of MEK, the MAPK kinase that phosphorylates and activates MAPK, and of a dominant-negative [Asn17]Ras mutant prevented the Et2Mal-induced accumulation of p21(waf1/cip1) mRNA. Similarly, the expression of MEK- and of [Asn17]Ras mutants decreased the 12-O-tetradecanoyl-phorbol 13-acetate (TPA)-mediated p21(waf1/cip1) induction. Furthermore, TPA-induced and serum-induced p21(waf1/cip1) mRNA accumulation was blocked by pretreating the cells with the antioxidant compound N-acetylcysteine, suggesting that oxidative stress is involved in these responses. p21(waf1/cip1) mRNA levels reached a maximum within 2 h...Continue Reading

References

Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R Gopalakrishna, W B Anderson
May 1, 1986·Archives of Biochemistry and Biophysics·B Halliwell, J M Gutteridge
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 1, 1981·Methods in Enzymology·J L PlummerJ R Bend
Nov 13, 1995·Biochemical and Biophysical Research Communications·Y OsawaM Akashi
Dec 8, 1995·The Journal of Biological Chemistry·T RussoE Appella
Aug 11, 1995·The Journal of Biological Chemistry·M AkashiH P Koeffler
Mar 1, 1995·Genes & Development·C E CanmanM B Kastan
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·M B DattoX F Wang
Feb 1, 1995·Mutation Research·J RemacleG Rao
Oct 1, 1994·European Journal of Biochemistry·R AmmendolaF Cimino
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Jun 7, 1996·Biochimica Et Biophysica Acta·T M Gottlieb, M Oren
Feb 1, 1996·Current Opinion in Genetics & Development·S Bates, K H Vousden
Aug 1, 1991·Trends in Cell Biology·R Schreck, P A Baeuerle

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Citations

Mar 13, 2010·In Vitro Cellular & Developmental Biology. Animal·Jurate SavickieneRuta Navakauskiene
Jun 25, 2002·Molecular Aspects of Medicine·Malcolm J JacksonSiân B Astley
Oct 12, 2000·Free Radical Biology & Medicine·S KohlhoffM Graessmann
Mar 4, 2000·Free Radical Biology & Medicine·R G Allen, M Tresini
Feb 4, 2006·Cell Death and Differentiation·K FigarellaM Duszenko
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·J SmithM Noble
Dec 10, 2002·Antioxidants & Redox Signaling·Nita N DeshpandeKathy K Griendling
Jan 15, 2005·Antioxidants & Redox Signaling·Michael A O'Reilly
Sep 26, 2009·Antioxidants & Redox Signaling·Matteo LandriscinaFranca Esposito
Feb 8, 2006·DNA and Cell Biology·Maryse BerthiaumeLuc Gaudreau
Apr 25, 2002·Molecular and Cellular Biology·Wengong WangMyriam Gorospe
May 20, 1999·Annual Review of Pharmacology and Toxicology·T P DaltonA Puga
Sep 3, 2002·American Journal of Respiratory and Critical Care Medicine·Katsuyuki TomitaIan M Adcock
Nov 7, 1998·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyW M Maniscalco
Jun 21, 2001·American Journal of Respiratory Cell and Molecular Biology·M A O'ReillyP C Keng
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Bernard M Fischer, Judith A Voynow
Jun 10, 2008·American Journal of Respiratory Cell and Molecular Biology·Hyun Jik KimJoo-Heon Yoon
Mar 31, 2009·American Journal of Respiratory Cell and Molecular Biology·Dongsun CaoJames M Samet
Feb 12, 2011·Cellular and Molecular Life Sciences : CMLS·Dong-Oh MoonGi-Young Kim
Aug 23, 2006·Oncogene·S G Menon, P C Goswami
Oct 1, 2004·Leukemia·E C Attar, D T Scadden
Dec 29, 2007·The Journal of Biological Chemistry·Jose L OlivaMarcelo G Kazanietz
Jun 25, 1998·The Journal of Cell Biology·S TanD Schubert
Aug 11, 2004·The Journal of Biological Chemistry·Shohreh AminiBassel E Sawaya
Nov 13, 2004·The Journal of Biological Chemistry·Severa BundaAleksander Hinek
Feb 29, 2000·The Journal of Biological Chemistry·S MilutinovicM Szyf
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·M A O'Reilly
Jun 5, 2002·World Journal of Gastroenterology : WJG·Jing-Yuan Fang, You-Yong Lu
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·V J Thannickal, B L Fanburg

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