Redox Modulating Factors Affect Longevity Regulation in Rotifers

The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Lilla MacsaiZsolt Datki

Abstract

Rotifers are microinvertebrate models to study the phylogenetically based mechanisms of aging. Our study aimed to develop a physiological system with electron deprivation via a chemical electron carrier/acceptor pair together with extreme caloric restriction (ECR). Middle-aged Philodina acuticornis rotifers were treated with combinations of phenazine methosulfate (PMS, electron carrier) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT, electron acceptor) for a period of 72 hours under total food deprivation (preselection). The ability of XTT to be reduced was confirmed both in vitro (with NADH) and in vivo (with live rotifers). Subsequently, the respective electron acceptor alone at a lower dose was administered in combination with ECR for several months on preselected survivors. We found that the longevity of rotifers markedly increased (4×) after PMS/XTT/total food deprivation preselection followed by XTT/ECR treatment. Ascorbic acid in equivalent concentrations caused similar but less pronounced tendencies. The synergistic effect of chemical electron deprivation and ECR caused delayed aging and the development of an outstanding phenotype that we refer to as "super rotifers," charact...Continue Reading

References

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Nov 28, 2016·Redox Biology·Akshaya ChandrasekaranJ Andrés Melendez

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Citations

Jun 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zsolt DatkiLaszlo Vecsei
Nov 9, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Lilla MácsaiZoltán P Zomborszki
Mar 18, 2020·Journal of Advanced Research·Arif Jamal SiddiquiWhitni Redman
Jan 6, 2021·Ecotoxicology and Environmental Safety·Zsolt DatkiZita Galik-Olah

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