Redox polymer and probe DNA tethered to gold electrodes for enzyme-amplified amperometric detection of DNA hybridization

Analytical Chemistry
Paul Kavanagh, Dónal Leech

Abstract

The detection of nucleic acids based upon recognition surfaces formed by co-immobilization of a redox polymer mediator and DNA probe sequences on gold electrodes is described. The recognition surface consists of a redox polymer, [Os(2,2'-bipyridine)2(polyvinylimidazole)(10)Cl](+/2+), and a model single DNA strand cross-linked and tethered to a gold electrode via an anchoring self-assembled monolayer (SAM) of cysteamine. Hybridization between the immobilized probe DNA of the recognition surface and a biotin-conjugated target DNA sequence (designed from the ssrA gene of Listeria monocytogenes), followed by addition of an enzyme (glucose oxidase)-avidin conjugate, results in electrical contact between the enzyme and the mediating redox polymer. In the presence of glucose, the current generated due to the catalytic oxidation of glucose to gluconolactone is measured, and a response is obtained that is binding-dependent. The tethering of the probe DNA and redox polymer to the SAM improves the stability of the surface to assay conditions of rigorous washing and high salt concentration (1 M). These conditions eliminate nonspecific interaction of both the target DNA and the enzyme-avidin conjugate with the recognition surfaces. The sens...Continue Reading

References

Sep 1, 1993·Analytical Chemistry·K M Millan, S R Mikkelsen
Jan 1, 1997·Bioconjugate Chemistry·S O KelleyM G Hill
Dec 9, 1998·Analytical Chemistry·A B SteelM J Tarlov
Aug 10, 2000·Nucleic Acids Research·J Wang
Oct 4, 2000·Nature Biotechnology·E M BoonJ K Barton
Oct 27, 2001·Biosensors & Bioelectronics·M I PividoriS Alegret
Jan 25, 2002·Analytical Chemistry·Charles N CampbellAdam Heller
Aug 15, 2002·Analytical Chemistry·Kenichi MaruyamaJunko Motonaka
Oct 24, 2002·Biosensors & Bioelectronics·Kazunori IkebukuroKoji Sode
Jan 10, 2003·Nucleic Acids Research·Christian ZwiebJacek Wower
Oct 2, 2003·Nature Biotechnology·T Gregory DrummondJacqueline K Barton
May 29, 2004·Analytical Chemistry·Elena DomínguezArántzazu Narváez
Aug 17, 2004·Biosensors & Bioelectronics·Guido CarpiniMarco Mascini
Jan 15, 2005·Analytical Chemistry·Seongpil HwangJuhyoun Kwak
Feb 19, 2005·Analytical and Bioanalytical Chemistry·Zhi-Ling ZhangHéctor D Abruña
Aug 16, 2005·Analytical Chemistry·Gilles MarchandFrédéric Ginot
Oct 13, 2005·FEMS Microbiology Reviews·Uta GasanovPhilip M Hansbro

❮ Previous
Next ❯

Citations

Jul 24, 2010·Analytical and Bioanalytical Chemistry·Dmitrii A GuschinWolfgang Schuhmann
Sep 8, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Marco FrasconiOded Shoseyov
May 15, 2010·Yi chuan = Hereditas·Ling-Wei WuZu-Hong Lu
May 17, 2013·Scientific Reports·Tobias KlampMarkus Sauer
May 24, 2018·Critical Reviews in Microbiology·Dharmendra Kumar SoniSuresh Kumar Dubey
Jul 23, 2008·The Analyst·Serge Cosnier, Pascal Mailley
Dec 16, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Xiang-Nan HuangHua-Bing Wang
Jun 1, 2013·Reports on Progress in Physics·Rajagopalan ThiruvengadathanShubhra Gangopadhyay
Mar 27, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Alejandra M RicciErnesto J Calvo
May 21, 2008·Journal of the American Chemical Society·Benoît LimogesBernd Schöllhorn
Apr 23, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Stephen A MerchantDavid W Schmidtke
Mar 5, 2009·Analytical Chemistry·Wei-Ching Liao, Ja-An Annie Ho
Dec 22, 2007·Chemical Reviews·Audrey SassolasLoïc J Blum
May 27, 2021·ACS Applied Materials & Interfaces·Carla Gonzalez-SolinoMirella Di Lorenzo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.