Redox properties of Arabidopsis cytochrome c6 are independent of the loop extension specific to higher plants

Biochimica Et Biophysica Acta
Jürgen WastlChristopher J Howe

Abstract

Cytochrome c6 (cytc6) from Arabidopsis differs from the cyanobacterial and algal homologues in several redox properties. It is possible that these differences might be due to the presence of a 12 amino acid residue loop extension common to higher plant cytc6 proteins. However, homology modelling suggests this is not the case. We report experiments to test if differences in biochemical properties could be due to this extension. Analysis of mutant forms of Arabidopsis cytc6 in which the entire extension was lacking, or a pair of cysteine residues in the extension had been exchanged for serine, revealed no significant effect of these changes on either the redox potential of the haem group or the reactivity towards Photosystem I (PSI). We conclude that the differences in properties are due to more subtle unidentified differences in structure, and that the sequence extension in the higher plant proteins has a function yet to be identified.

References

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Jun 7, 2002·Trends in Plant Science·Jürgen WastlChristopher J Howe
May 30, 2003·The Journal of Biological Chemistry·Martin WeigelDario Leister
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Citations

Jul 28, 2007·Extremophiles : Life Under Extreme Conditions·Keiko OgawaYoshihiro Sambongi
Jan 27, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Badri S RajagopalJonathan A R Worrall
Jun 17, 2006·FEBS Letters·Hirotaka ChidaTadatake Oku
Apr 4, 2006·FEBS Letters·Beatrix G Schlarb-RidleyDerek S Bendall
Nov 17, 2011·Biochimica Et Biophysica Acta·Jody M MasonJonathan A R Worrall
Dec 1, 2005·Journal of Experimental Botany·Christopher J HoweDerek S Bendall
Nov 22, 2008·Biochemical Society Transactions·Jonathan A R WorrallChristopher J Howe
Apr 29, 2008·Biochemistry·Wojciech BialekAndrzej Szczepaniak

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