Redox regulation of Cdc25C.

The Journal of Biological Chemistry
Pavel A Savitsky, Toren Finkel

Abstract

The Cdc25 family of dual specific phosphatases are critical components of cell cycle progression and checkpoint control. Certain stresses such as ultraviolet light stimulate the rapid and selective destruction of Cdc25A protein through a Chk1 protein kinase-dependent pathway. We demonstrate that in contrast to cellular stresses previously examined, hydrogen peroxide exposure affects Cdc25C but not Cdc25A levels. Pharmacological inhibition of Chk1 activity or a mutant of Cdc25C that lacks the Chk1 phosphorylation site still undergoes degradation in response to oxidants. We also demonstrate that in vitro hydrogen peroxide stimulates an intramolecular disulfide bond between the active site cysteine at position 377 and another invariant cysteine at position 330. The in vivo stability of Cdc25C is substantially reduced by the mutation of either of these two cysteine residues. In contrast, a double (C2) mutant of both cysteine 330 and cysteine 377 results in a protein that is more stable than wild type Cdc25C and is resistant to oxidative stress-induced degradation. In addition, the C2 mutant, which is unable to form an intramolecular disulfide bond, has reduced binding to 14-3-3 in vitro and in vivo. These results suggest that oxida...Continue Reading

References

May 18, 1999·Molecular and Cellular Biology·S N DalalJ A DeCaprio
Feb 22, 2000·The Journal of Biological Chemistry·P R GravesH Piwnica-Worms
May 10, 2000·Trends in Cell Biology·B G den Boer, J A Murray
Feb 16, 2002·Cellular and Molecular Life Sciences : CMLS·A J Obaya, J M Sedivy

❮ Previous
Next ❯

Citations

Jul 17, 2004·Histochemistry and Cell Biology·George L King, Mary R Loeken
Apr 24, 2009·Biological Trace Element Research·Wayne Chris HawkesKevin Dawson
Jul 11, 2003·Free Radical Biology & Medicine·Kai ChenJohn F Keaney
Mar 22, 2003·Current Opinion in Cell Biology·Toren Finkel
Sep 19, 2003·Trends in Biochemical Sciences·Paola Chiarugi, Paolo Cirri
Jan 19, 2011·The Journal of Biological Chemistry·Min HuangBeverly S Mitchell
Aug 9, 2008·The Journal of Biological Chemistry·Nirupama Gupta, Stephen W Ragsdale
Jan 15, 2005·Antioxidants & Redox Signaling·Irfan RahmanH J Forman
May 14, 2005·Antioxidants & Redox Signaling·Annette Salmeen, David Barford
May 14, 2005·Antioxidants & Redox Signaling·Paola Chiarugi, Elisa Giannoni
May 14, 2005·Antioxidants & Redox Signaling·Emily C Rothstein, Pamela A Lucchesi
Sep 22, 2006·Antioxidants & Redox Signaling·Janet V Cross, Dennis J Templeton
Nov 23, 2006·Antioxidants & Redox Signaling·Paola Chiarugi, Francesca Buricchi
Aug 19, 2008·Antioxidants & Redox Signaling·Shane R ThomasGrant R Drummond
Aug 19, 2008·Antioxidants & Redox Signaling·Saghi Ghaffari
Jun 10, 2009·Antioxidants & Redox Signaling·Ehab H SarsourPrabhat C Goswami
Sep 10, 2009·Antioxidants & Redox Signaling·Margarete LukoszJudith Haendeler
Jun 30, 2009·Antioxidants & Redox Signaling·Gillian GroegerThomas G Cotter
Aug 5, 2009·Antioxidants & Redox Signaling·Shazib PervaizSaghi Ghaffari
Aug 23, 2011·Journal of Biochemistry·Arne OstmanFrank-D Böhmer
May 5, 2007·Nucleic Acids Research·Cyril M SandersAlfred A Antson
Mar 19, 2004·Biology of Reproduction·Manabu OzawaYukio Kanai
Apr 7, 2011·Science Signaling·J Jefferson P Perry, John A Tainer
Sep 17, 2008·Cancer Research·Robert J Tomko, John S Lazo
Jun 20, 2012·Cancer Research·Ehab H SarsourPrabhat C Goswami
Feb 9, 2005·The Journal of Reproduction and Development·Takaya MatsuzukaYukio Kanai
Apr 22, 2005·Biological Chemistry·Andreas Barthel, Lars-Oliver Klotz
Sep 12, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Anna AjdukMarek Maleszewski
Jan 5, 2014·Antioxidants & Redox Signaling·Raymond Liang, Saghi Ghaffari
Mar 28, 2008·Proceedings of the National Academy of Sciences of the United States of America·Xiaoming ZhouMaurice B Burg
Mar 8, 2013·Biochimica Et Biophysica Acta·Rosalind Brigham Penney, Deodutta Roy
Oct 12, 2013·Antioxidants & Redox Signaling·Jeroen FrijhoffArne Ostman
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xiaona LiuBiplab Dasgupta
Feb 15, 2012·Journal of Amino Acids·Noriyuki Nagahara
May 14, 2005·Antioxidants & Redox Signaling·Johannes Rudolph
Oct 21, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Tong-Tong LiuKui Wang
Sep 17, 2004·Endothelium : Journal of Endothelial Cell Research·Kai Chen, John Keaney

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.