Redox regulation of microRNAs in endometriosis-associated pain.

Redox Biology
Kristeena Ray WrightNalini Santanam

Abstract

Endometriosis is a chronic, painful condition with unknown etiology. A differential expression of microRNAs in the endometriotic tissues from women with endometriosis with pain compared to those without suggested a plausible role for miRNA or epigenetic mechanisms in the etiology of endometriotic pain. The peritoneal milieu is involved in maintenance of endometriotic lesion and nociception. We recently showed the mechanistic role for oxidized-lipoproteins (ox-LDLs) present in peritoneal fluid (PF) in endometriosis and pain. We explored the possibility of ox-LDLs modulating the expression of miRNAs in a manner similar to PF from women with endometriosis. Expression levels of miRNAs and their predicted nociceptive and inflammatory targets were determined in PF and ox-LDL treated human endometrial cell-lines. Samples from IRB-approved and consented patients with and without endometriosis or pain were used. These were compared to endometrial cell-lines treated with various forms of oxidized-lipoproteins. RNA (including miRNAs) were isolated from treated endometrial cells and expression levels were determined using commercial miRNome arrays. Cell lysates were used in immunoblotting for inflammatory proteins using a protein array. Tw...Continue Reading

Citations

Feb 6, 2019·Biology of Reproduction·Sarah Bjorkman, Hugh S Taylor
May 10, 2020·Anesthesia and Analgesia·Juan P CataHolger K Eltzschig
Feb 16, 2018·Pain·Claudia SommerNurcan Üçeyler
Oct 24, 2017·Oxidative Medicine and Cellular Longevity·Gennaro ScutieroLuigi Nappi
Nov 17, 2019·International Journal of Molecular Sciences·Josep Marí-AlexandreJuan Gilabert-Estellés
Mar 1, 2019·International Journal of Molecular Medicine·Xiaobing YuBaolin Wu
Aug 14, 2020·Journal of Translational Medicine·Helena MalvezziCarla de Azevedo Piccinato
Aug 28, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jan 11, 2019·BioMed Research International·Zhuo-Ying TaoDong-Yuan Cao
Nov 3, 2020·Frontiers in Cellular Neuroscience·Jessica MaddernStuart M Brierley
Feb 4, 2021·Experimental and Molecular Pathology·Jéssica Vilarinho CardosoJamila Alessandra Perini
Apr 4, 2021·International Journal of Molecular Sciences·Sarah BruntyNalini Santanam

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Methods Mentioned

BETA
PCR
protein array

Software Mentioned

TargetScan
GraphPad
SA Biosciences
IPA
Prism

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