Redox regulation of the tumor suppressor PTEN by glutathione

FEBS Letters
Yujeong KimSeung-Rock Lee

Abstract

Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expressed in Saccharomyces cerevisiae was reversibly oxidized by hydrogen peroxide and reduced by cellular reductants. Reduction of hPTEN was delayed in each of S. cerevisiae gsh1Delta and gsh2Delta mutants. Expression of gamma-glutamylcysteine synthetase Gsh1 in the gsh1Delta mutant rescued regeneration rate of hPTEN. Oxidized hPTEN was reduced by glutathione in a concentration- and time-dependent manner. Glutathionylated PTEN was detected. Incubation of 293T cells with BSO and knockdown expression of GCLc in HeLa cells by siRNA resulted in the delay of reduction of oxidized PTEN. Also, in HeLa cells transfected with GCLc siRNA, stimulation with epidermal growth factor resulted in the increase of oxidized PTEN and phosphorylation of Akt. These results suggest that the reduction of oxidized hPTEN is mediated by glutathione.

References

Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L C Cantley, B G Neel
Jan 5, 2002·Physiological Reviews·Wulf Dröge
Mar 28, 2002·The Journal of Biological Chemistry·Seung-Rock LeeSue Goo Rhee
Jun 21, 2002·Methods in Enzymology·Fred Sherman
Oct 9, 2003·The EMBO Journal·Nick R LeslieC Peter Downes
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jaeyul KwonSue Goo Rhee
Jun 22, 2005·Molecular Pharmacology·Chang-Xi YuA Richard Whorton
Jul 5, 2005·Developmental Biology·Beom-Jun KimSa-Ouk Kang
Feb 23, 2007·Yeast·Ivan SadowskiJennifer Parent
Mar 10, 2007·Cellular Signalling·Sarah H RossNick R Leslie

❮ Previous
Next ❯

Citations

Mar 5, 2011·Biochemical and Biophysical Research Communications·Yujeong KimSeung-Rock Lee
Feb 19, 2013·Biochimica Et Biophysica Acta·Pietro Ghezzi
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rafael Pulido
May 11, 2017·International Journal of Molecular Sciences·Ying ZhangSeung-Rock Lee
Apr 25, 2021·Archives of Biochemistry and Biophysics·Debojyoti PalSantosh K Sandur
Apr 25, 2021·Redox Biology·Claudia Lennicke, Helena M Cochemé

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.