Redox-sensitive YFP sensors monitor dynamic nuclear and cytosolic glutathione redox changes.

Free Radical Biology & Medicine
Michèle DardalhonMeng-Er Huang

Abstract

Intracellular redox homeostasis is crucial for many cellular functions but accurate measurements of cellular compartment-specific redox states remain technically challenging. To better characterize redox control in the nucleus, we targeted a yellow fluorescent protein-based redox sensor (rxYFP) to the nucleus of the yeast Saccharomyces cerevisiae. Parallel analyses of the redox state of nucleus-rxYFP and cytosol-rxYFP allowed us to monitor distinctively dynamic glutathione (GSH) redox changes within these two compartments under a given condition. We observed that the nuclear GSH redox environment is highly reducing and similar to the cytosol under steady-state conditions. Furthermore, these sensors are able to detect redox variations specific for their respective compartments in glutathione reductase (Glr1) and thioredoxin pathway (Trr1, Trx1, Trx2) mutants that have altered subcellular redox environments. Our mutant redox data provide in vivo evidence that glutathione and the thioredoxin redox systems have distinct but overlapping functions in controlling subcellular redox environments. We also monitored the dynamic response of nucleus-rxYFP and cytosol-rxYFP to GSH depletion and to exogenous low and high doses of H₂O₂ bursts....Continue Reading

References

May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G BellomoS Orrenius
Apr 1, 1982·Molecular and Cellular Biology·A E Wheals
May 20, 1999·The Journal of Cell Biology·F MadeoK U Fröhlich
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Meng-Er HuangRichard D Kolodner
Jul 28, 2004·The Journal of Cell Biology·Henrik ØstergaardJakob R Winther
Dec 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Cary D AustinSuzie J Scales
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·Jason M HansenDean P Jones
Mar 17, 2006·Free Radical Biology & Medicine·Marcelo G BoniniRonald P Mason
Nov 28, 2007·Biochimica Et Biophysica Acta·H Reynaldo López-Mirabal, Jakob R Winther
Jan 8, 2008·Biochimica Et Biophysica Acta·Enrique HerreroElisa Cabiscol
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rosa E HansenJakob R Winther
Feb 24, 2009·Molecular Aspects of Medicine·Federico V PallardóJosé Viña
Jul 31, 2009·PloS One·Jelena MarkovicFederico V Pallardó
Aug 8, 2009·Cell Death and Differentiation·R Franco, J A Cidlowski
Dec 10, 2009·Nature Reviews. Molecular Cell Biology·Joseph R CaseyJohn Orlowski
Jan 22, 2010·Antioxidants & Redox Signaling·Andreas J Meyer, Tobias P Dick
Mar 10, 2010·Antioxidants & Redox Signaling·Young-Mi Go, Dean P Jones
May 26, 2010·The Journal of Cell Biology·Anne ChadrinValérie Doye
Oct 5, 2010·Free Radical Biology & Medicine·Anita AyerGabriel G Perrone
Oct 26, 2010·Free Radical Biology & Medicine·Xiaoguang OuyangThomas R Sutter
Mar 23, 2011·Biochimica Et Biophysica Acta·Rick OrijGertien J Smits
Jun 23, 2011·Journal of Cell Science·Marcel van LithNeil J Bulleid

❮ Previous
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Citations

Oct 15, 2013·Trends in Biochemical Sciences·J F WoolleyT G Cotter
Dec 4, 2012·Antioxidants & Redox Signaling·Michel B ToledanoAeid Igbaria
Feb 4, 2014·Antioxidants & Redox Signaling·Jun Lu, Arne Holmgren
Dec 18, 2012·Nature Chemical Biology·Bruce MorganTobias P Dick
Nov 8, 2015·Free Radical Biology & Medicine·Pedro Diaz-VivancosChristine H Foyer
Mar 1, 2016·Free Radical Biology & Medicine·Narciso CoutoJill Barber
Feb 6, 2014·Biotechnology Journal·Sandrine Pouvreau
Jul 31, 2013·Free Radical Biology & Medicine·Agata Banach-LatapyMeng-Er Huang
Oct 17, 2012·Biochimica Et Biophysica Acta·José Luis García-GiménezFederico V Pallardó
Oct 23, 2013·Free Radical Biology & Medicine·Elie HatemStéphane Chédin
Apr 14, 2015·Antioxidants & Redox Signaling·Markus SchwarzländerBruce Morgan
May 22, 2016·Biochimica Et Biophysica Acta·Phillip A WagesJames M Samet
May 26, 2017·Antioxidants & Redox Signaling·Marcel Deponte
Aug 8, 2014·Metallomics : Integrated Biometal Science·Eman A AkamElisa Tomat
Jun 1, 2017·Antioxidants & Redox Signaling·Gaetano CalabreseJan Riemer
Dec 4, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuta Hatori
Apr 9, 2017·Analytical and Bioanalytical Chemistry·Shakir ShakirGiovanni Chiappetta
Apr 11, 2018·Antioxidants & Redox Signaling·Juan I SbodioBindu D Paul
Nov 5, 2020·International Journal of Molecular Sciences·Alexander I KostyukDmitry S Bilan

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