Redox speciation of iron, manganese, and copper in cerebrospinal fluid by strong cation exchange chromatography - sector field inductively coupled plasma mass spectrometry

Analytica Chimica Acta
Nikolay SolovyevBernhard Michalke

Abstract

A new method of simultaneous redox speciation of iron (II/III), manganese (II/III), and copper (I/II) in cerebrospinal fluid (CSF) has been designed. For the separation of redox species strong cation exchange chromatography (SCX) with isocratic elution was employed. Species were detected using inductively coupled plasma sector field mass spectrometry (ICP-sf-MS), operating at medium resolution. The following parameters were optimized: analytical column, eluent composition and pH, CSF injection volume and dilution factor. Analytical column Dionex IonPac CS5A RFIC 4*250 mm was found to retain and separate species of interest the most effectively under the isocratic elution with a buffer, containing 50 mM ammonium citrate, 7.0 mM pyridine-2,6-dicarboxylic acid at pH = 4.2 and flow rate of 0.8 L min-1. Injection volume of 50 μL with CSF sample dilution of 1/3 (v/v) with the eluent was shown to result in minimal matrix suppression. For species identification, retention time matching with standards was used. The stability of metalloproteins (ferritin, transferrin, and ceruloplasmin) under elution conditions was evaluated. For the quantification of redox species, external calibration was employed. To avoid column contamination, a blan...Continue Reading

Citations

Feb 15, 2018·Biological Trace Element Research·Evgenii J DrobyshevVadim A Kashuro
May 21, 2020·PloS One·Nico LaurHans-Peter Deigner
Aug 14, 2020·Metallomics : Integrated Biometal Science·C Derrick QuarlesM Paul Field
Oct 4, 2018·Current Environmental Health Reports·Marco VincetiLauren A Wise
Jun 30, 2019·Hormones : International Journal of Endocrinology and Metabolism·Nikolay Solovyev
Nov 25, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Radosław Kotuniak, Wojciech Bal

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