Redox state affects fecundity and insecticide susceptibility in Anopheles gambiae

Scientific Reports
Cody J Champion, Jiannong Xu

Abstract

Redox reactions play a central role in the metabolism of an organism. It is vital to maintain redox homeostasis in response to the fluctuation of redox shift in various biological contexts. NADPH-dependent reducing capacity is one of the key factors contributing to the redox homeostasis. To understand the redox capacity and its impact on mosquito fecundity and susceptibility to insecticides in Anopheles gambiae, we examined the dynamics of elevated oxidative state via induction by paraquat (PQ) and the inhibition of NADPH regeneration by 6-aminonicotinamide (6AN). In naïve conditions, inherent oxidative capacity varies between individuals, as measured by GSSG/GSH ratio. The high GSSG/GSH ratio was negatively correlated with fecundity. Both PQ and 6AN feeding increased GSSG/GSH ratio and elevated protein carbonylation, a marker of oxidative damage. Both pro-oxidants lowered egg production. Co-feeding the pro-oxidants with antioxidant lycopene attenuated the adverse effects on fecundity, implying that oxidative stress was the cause of this phenotype. Pre-feeding with 6AN increased insecticide susceptibility in DDT resistant mosquitoes. These results indicate that oxidative state is delicate in mosquitoes, manipulation of NADPH po...Continue Reading

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Citations

May 28, 2020·Frontiers in Microbiology·Shicheng ChenEdward D Walker
Sep 12, 2020·Parasites & Vectors·Eunice Oluwatobiloba AdedejiEzekiel Adebiyi
Mar 26, 2021·Scientific Reports·Lue SunTakashi Moritake
Sep 7, 2021·Frontiers in Cellular and Infection Microbiology·Ana Cristina S BombaçaRubem F S Menna-Barreto

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Methods Mentioned

BETA
dissection
PCR

Software Mentioned

GraphPad
GraphPad Prism
CFX Connect Real - Time System
R

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