Reduced 11beta-hydroxysteroid dehydrogenase activity in the remaining kidney following nephrectomy

Endocrinology
G EscherF J Frey

Abstract

Intracellular access of steroids to gluco- and mineralocorticoid receptors is regulated by reduced 11beta-hydroxysteroid dehydrogenase (OHSD) 1 and 2. These enzymes convert active 11beta-OH-steroids into inactive 11-keto-steroids. The purpose of the present study was to establish whether the 11beta-OHSD1 and 11beta-OHSD2 are modulated in the remnant kidney 24 h or 14 days after uninephrectomy (UNX) in rats. Overall, 11beta-OHSD activity was analyzed by measuring the ratio of the exogenous 11beta-OH-steroid prednisolone to its 11-keto metabolite prednisone in vivo in kidney tissue using high performance liquid chromatography. To determine which isoenzyme accounts for the changed activity 24 h after UNX, the oxidation and reduction attributable to 11beta-OHSD1 and oxidation to 11beta-OHSD2 were analyzed in total renal extracts and in isolated glomeruli, proximal convoluted tubules (PCT), cortical ascending limbs, and cortical convoluted tubules (CCT). The messenger RNA content of 11beta-OHSD1 and 11beta-OHSD2 was measured by RT-PCR in renal tissues and single segments, using glyceraldehyde-3-phosphate-dehydrogenase as an internal standard. Protein amounts of 11beta-OHSD1 and 11beta-OHSD2 were assessed by Western blot. The prednis...Continue Reading

References

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Citations

Jun 13, 2002·European Journal of Clinical Investigation·A AudigéB Vogt
Jun 17, 2004·Current Opinion in Nephrology and Hypertension·Felix J FreyBrigitte M Frey
Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D AckermannF J Frey
Jul 2, 2014·Hypertension·Mina RezaeiBrigitte M Frey
Nov 7, 2001·FEBS Letters·C D HeinigerB M Frey
Feb 25, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Felix J Frey
Mar 29, 2007·American Journal of Physiology. Renal Physiology·Kristie UsaMingyu Liang

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