Reduced activity of Arabidopsis thaliana HMT2, a methionine biosynthetic enzyme, increases seed methionine content

The Plant Journal : for Cell and Molecular Biology
Minsang LeeGeorg Jander

Abstract

In the S-methylmethionine cycle of plants, homocysteine methyltransferase (HMT) catalyzes the formation of two molecules of methionine from homocysteine and S-methylmethionine, and methionine methyltransferase (MMT) catalyzes the formation of methionine from S-methylmethionine using S-adenosylmethionine as a methyl group donor. Somewhat surprisingly, two independently isolated knockdown mutations of HMT2 (At3g63250), one of three Arabidopsis thaliana genes encoding homocysteine methyltransferase, increased free methionine abundance in seeds. Crosses and flower stalk grafting experiments demonstrate that the maternal genotype at the top of the flower stalk determines the seed S-methylmethionine and methionine phenotype of hmt2 mutants. Uptake, transport and inter-conversion of [(13)C]S-methylmethionine and [(13)C]methionine in hmt2, mmt and wild-type plants show that S-methylmethionine is a non-essential intermediate in the movement of methionine from vegetative tissue to the seeds. Together, these results support a model whereby elevated S-methylmethionine in hmt2 vegetative tissue is transported to seeds and either directly or indirectly results in the biosynthesis of additional methionine. Manipulation of the S-methylmethioni...Continue Reading

References

Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Apr 20, 2001·The Plant Journal : for Cell and Molecular Biology·P RanochaA D Hanson
Jun 18, 2002·Plant Physiology·Georg JanderRobert L Last
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Citations

Nov 26, 2009·The Plant Journal : for Cell and Molecular Biology·Liping GuRobert L Last
Apr 23, 2010·The Plant Journal : for Cell and Molecular Biology·Steven D Rounsley, Robert L Last
Jul 22, 2014·The Biochemical Journal·Louis M T BradburyAndrew D Hanson
Mar 10, 2015·Frontiers in Plant Science·Sudhakar PanduranganFrédéric Marsolais
Jan 7, 2016·Annual Review of Plant Biology·Gad GaliliAlisdair R Fernie
Apr 21, 2018·International Journal of Molecular Sciences·Man ZhaoXiangxian Ying
Mar 27, 2019·Journal of Experimental Botany·Rachel AmirYael Hacham
Jan 1, 2009·The Arabidopsis Book·Georg Jander, Vijay Joshi
Apr 4, 2017·Journal of Experimental Botany·Rikard Fristedt
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder

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