Abstract
CD36 is an 88-kDa glycoprotein expressed on platelets and monocyte/macrophages (Mphi). CD36 is a multifunctional receptor for collagen, thrombospondin, oxidized low density lipoproteins (LDL), and long-chain fatty acids. The present study was performed to investigate whether CD36 can function as an adhesion molecule which is involved in mediating human macrophages (Mphi) adhesion to type I collagen in vitro. The Mphi of human CD36-deficient as well as normal control subjects were isolated and cultured on the multi-well plates coated with type I collagen, a natural ligand for CD36. Up to 2 h of incubation, the Mphi from CD36-deficient patients showed almost a approximately 55% decrease in adhesion to type I collagen in comparison to those from controls (P < 0.01). However, there was no significant difference in the adhesion thereafter. Furthermore, the addition of antibody against CD36 into the media of control Mphi significantly inhibited the adhesion by approximately 50% (P < 0.05). The addition of oxidized LDL (OxLDL) did not alter adhesion of Mphi from both CD36-deficient and controls. These data suggest that CD36 is involved in the adhesion of Mphi to type I collagen, especially in the early stage of adhesion.
References
Dec 1, 1992·Journal of Leukocyte Biology·R A MufsonM Meltzer
Jul 1, 1991·Scandinavian Journal of Immunology·J ViherluotoM Hurme
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M M HuangJ S Brugge
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M KirsteinH Vlassara
Oct 1, 1989·Analytical Biochemistry·W KuengU Eppenberger
Jan 1, 1989·Vox Sanguinis·H IkedaS Sekiguchi
Nov 1, 1989·The Journal of Clinical Investigation·M MoroiK Shinmyozu
Nov 15, 1986·Analytical Biochemistry·R J GilliesM Denton
Mar 1, 1995·The Journal of Clinical Investigation·H KashiwagiY Matsuzawa
Oct 1, 1995·The Journal of Clinical Investigation·S NozakiK Kameda-Takemura
Aug 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·H KashiwagiY Matsuzawa
Jan 14, 1999·The New England Journal of Medicine·R Ross
May 14, 1999·Arteriosclerosis, Thrombosis, and Vascular Biology·A NakataY Matsuzawa
May 20, 1999·Circulation·G K SukhovaP Libby
Jun 22, 1999·The New England Journal of Medicine·G D Sloop
Feb 25, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·B B GowenE P Mayer
Aug 11, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·M JanabiY Matsuzawa
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A PainD J Roberts
Citations
Apr 26, 2002·Immunology·Shouwei Han, Neil Sidell
Jan 31, 2012·Current Atherosclerosis Reports·Tanu GoyalJawahar L Mehta
Feb 6, 2018·Oncogene·Andras LadanyiErnst Lengyel
May 4, 2006·Molecular and Cellular Biochemistry·Shizuya YamashitaNaohiko Sakai
Jun 9, 2020·Frontiers in Oncology·Erica J Hoffmann, Suzanne M Ponik