Reduced adipogenesis after lung tumor exosomes priming in human mesenchymal stem cells via TGFβ signaling pathway

Molecular and Cellular Biochemistry
Shihua WangRobert Chunhua Zhao

Abstract

A key feature of cancer cachexia is the loss of adipose tissue, mainly due to increased lipolysis and an impairment of adipogenesis. Recent findings have shown that cancer exosomes promoted lipolysis in adipose tissue. However, effects of cancer exosomes on adipogenesis were not reported. In this study, we found that lung cancer exosomes could be internalized by human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) and significantly inhibited hAD-MSC adipogenesis as demonstrated by Oil Red O staining and decreased expression of adipogenic-specific genes. Specifically, TGFβ signaling pathway was demonstrated to be involved in the inhibitive effects of lung cancer exosomes on hAD-MSC adipogenesis. Additionally, TGFβ was detected in A549 exosomes. Herein, this study reports that the effect of lung cancer cell exosomes on hAD-MSC adipogenic differentiation was mediated by TGFβ signaling pathway and suggests the involvement of cancer exosomes in weight loss of cancer cachexia.

References

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Citations

Nov 22, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Lucía Robado de LopeHéctor Peinado
Feb 27, 2019·International Journal of Cancer. Journal International Du Cancer·Lei ShenGuohao Wu
Aug 23, 2019·Journal of Cachexia, Sarcopenia and Muscle·Rebeka TomasinMárcia Regina Cominetti
Aug 5, 2020·Cell Communication and Signaling : CCS·Wei WangLi Qin
Dec 5, 2019·International Journal of Molecular Sciences·Mercedes HerreraCristina Peña
Sep 23, 2018·Biochemical Society Transactions·Sai V ChittiSuresh Mathivanan
Jul 7, 2019·International Journal of Molecular Sciences·Maria Giovanna ScioliAugusto Orlandi
Feb 2, 2021·Medicinal Research Reviews·Milena DeptułaMichał Pikuła
May 18, 2021·Non-coding RNA Research·Guannan DuYi Li
Jul 10, 2021·Frontiers in Cell and Developmental Biology·Yuxuan ZhongJun Fan
Aug 25, 2021·Technology in Cancer Research & Treatment·Qian ZhangHuanliang Liu

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Methods Mentioned

BETA
light microscopy
Assay
transmission electron microscopy
PCR
Protein Assay
confocal microscopy
ELISA

Software Mentioned

SPSS Statistics

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