Reduced antigen production by hepatitis B virus harbouring nucleotide deletions in the overlapping X gene and precore-core promoter

The Journal of General Virology
K Moriyama

Abstract

Hepatitis B virus (HBV) genomes with deletions in the precore-core (preC-C) promoter have been detected in HBV infections without serological markers. To address whether the mutations are responsible for the reduced production of virus antigenes, either an 8 bp (8d, position 1763 to 1770) or a 20 bp (20d, 1753 to 1772) deletion was created in a wild-type (wt) HBV clone. Both mutations cause premature termination of the overlapping X ORF. When introduced into HepG2 cells, both mutants produced reduced amounts of HBsAg, HBcAg and HBeAg, but released the same or more virion-associated DNA compared with the wt. A co-transfection of the 20d mutant with a small amount of intact X gene resulted in a 3-fold increase of HBcAg production compared to transfection with either the 20d or wt alone. When the promoter region was cloned into CAT plasmids, the 8d preC promoter showed weak activity and its initiation site was shifted 6 to 10 bp downstream. The preC promoter activity of 20d was not detectable by CAT ELISA and 5' RACE. The levels of C transcripts of both mutants were higher than that of the wt, and their start sites were not altered. Therefore, the deletions cause the reduction of HBsAg, HBcAg and HBeAg although the mutant viruses ...Continue Reading

Citations

Nov 20, 2015·The Journal of General Virology·Leandro R JonesJorge Quarleri
Mar 8, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A ErhardtD Häussinger
Mar 18, 2008·The Journal of General Virology·Saffie JammehPeter Karayiannis
Aug 13, 1998·Journal of Medical Virology·E D McIntoshY E Cossart
Oct 10, 1998·European Journal of Clinical Investigation·F Torre, N V Naoumov
Jun 15, 2011·Carcinogenesis·Teresa PollicinoGiovanni Raimondo
May 5, 2017·PloS One·Ina SevicRodolfo Hector Campos
Dec 22, 1999·Journal of Viral Hepatitis·A Kramvis, M C Kew
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Petra PreikschatHelga Meisel
Sep 13, 2002·Journal of Medical Virology·Talal A Sallam, C Y William Tong
Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W E Delaney, H C Isom
May 11, 2002·Journal of Cellular and Molecular Medicine·C Kreutz
Jun 18, 2003·The Journal of General Virology·Kosei MoriyamaYasuyuki Fukumaki
Apr 8, 2016·World Journal of Gastroenterology : WJG·Hui-Lan ZhuZhen-Hua Zhang
Sep 10, 1999·Reviews in Medical Virology·S L NguiC G Teo
Apr 10, 2007·Acta Biochimica Et Biophysica Sinica·Ping'an ZhuLin Song
Nov 13, 2018·Archives of Virology·Ina SevicRodolfo Héctor Campos

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.