Reduced-bore monolithic silica column modified with C8-TEOS for reversed-phase electrochromatography

Journal of Separation Science
Qishu QuYou-zhao He

Abstract

Monolithic silica columns of 2.7 mm ID were prepared and derivatized with C8-TEOS and TEOS by on-column sol-gel reaction. These C8 large diameter monolithic silica columns gave 21 000 theoretical plates for aromatic hydrocarbons in 60% acetonitrile and 40% Tris-HCI buffer. The surface areas as well as the separation reproducibility were improved on coating by the sol-gel approach. Joule heating was greatly reduced by using monolithic columns to which fine quartz sand had been added during column preparation. Since this is a preliminary investigation on a monolithic column with such a large inner diameter, the separation efficiency was not so high as that presently achieved in normal capillary electrochromatography (CEC). However, use of the columns improved sample loadability and concentration detectability of electrochromatography, and semi-preparative separations could be performed.

References

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Citations

Aug 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yugao GuoJames Jianmin Bao
Jun 1, 2007·Journal of Separation Science·Guijie ZhuYukui Zhang
May 8, 2007·Electrophoresis·Ruijuan YuanJames J Bao
Oct 1, 2010·Electrophoresis·You-Xin LiJames Jianmin Bao

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