Reduced endothelial nitric oxide synthase expression and production in human atherosclerosis

Circulation
B S OemarT F Lüscher

Abstract

NO regulates vascular tone and structure, platelets, and monocytes. NO is synthesized by endothelial NO synthase (eNOS). Endothelial dysfunction occurs in atherosclerosis. With a porphyrinic microsensor, NO release was measured in atherosclerotic human carotid arteries and normal mammary arteries obtained during surgery. eNOS protein expression was analyzed by immunohistochemistry. In normal arteries, the initial rate of NO release after stimulation with calcium ionophore A23187 (10 micromol/L) was 0.42+/-0.05 (micromol/L)/s (n=10). In contrast, the initial rate of NO release was markedly reduced in atherosclerotic segments, to 0.08+/-0.04 (micromol/L)/s (n=10, P<0.0001). NO peak concentration in normal arteries was 0.9+/-0.09 micromol/L (n=10) and in atherosclerotic segments, 0.1+/-0.03 micromol/L (n=10, P<0.0001). Reduced NO release in atherosclerotic segments was accompanied by marked reduction of immunoreactive eNOS in luminal endothelial cells, although specific endothelial cell markers (CD31) were present (n=13). Endothelial cells of vasa vasorum of atherosclerotic segments, however, remained positive for eNOS, as was the endothelium of normal arteries. In clinically relevant human atherosclerosis, eNOS protein expression...Continue Reading

Associated Clinical Trials

References

Sep 1, 1992·The Journal of Clinical Investigation·J P CookeM E Billingham
Dec 1, 1990·The Journal of Clinical Investigation·R L MinorD G Harrison
Aug 25, 1988·The New England Journal of Medicine·T F LüscherE Weber
Jan 1, 1993·Surgical and Radiologic Anatomy : SRA·J Henriquez-PinoJ C Prates
Aug 15, 1996·The Journal of Clinical Investigation·M R TschudiT F Lüscher
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·B V KhanR M Medford
Jan 1, 1997·The Journal of Clinical Investigation·E StroesT Rabelink
Feb 1, 1997·Journal of the American College of Cardiology·A A QuyyumiR O Cannon
Jan 1, 1997·Annual Review of Medicine·J P Cooke, V J Dzau

❮ Previous
Next ❯

Citations

Feb 24, 2000·The Journal of Pathology·H Li, U Förstermann
Mar 16, 2007·Basic Research in Cardiology·Felix C TannerThomas F Lüscher
Sep 1, 2004·Histochemistry and Cell Biology·Barbara VoetschJoseph Loscalzo
Dec 19, 2007·Pharmaceutical Research·R Preston MasonTadeusz Malinski
Apr 5, 2001·Current Atherosclerosis Reports·J P Cooke, R K Oka
Apr 8, 2006·Current Hypertension Reports·Zhihong Yang, Xiu-Fen Ming
May 10, 2005·The American Journal of Cardiology·Todd J AndersonMichael J Curtis
Mar 24, 2004·Archives of Medical Research·Armando Rojas Rubio, Miguel A Morales-Segura
Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Santhanam A V Raghavan, Madhu Dikshit
May 9, 2003·The Annals of Thoracic Surgery·Takashi UedaAkira Yoshioka
May 3, 2003·Biochemical and Biophysical Research Communications·Christine BarandierZhihong Yang
Nov 27, 1999·Life Sciences·D GattulloG Losano
Jul 27, 2002·Journal of the American College of Cardiology·Maria G ModenaRosario Rossi
Sep 14, 1999·Journal of the American College of Cardiology·T J Anderson
Dec 19, 2000·Free Radical Biology & Medicine·D R Janero, J F Ewing
Oct 17, 2002·Nitric Oxide : Biology and Chemistry·Huige LiUlrich Förstermann
Dec 14, 2006·Prostaglandins & Other Lipid Mediators·Rita K UpmacisDavid P Hajjar
Dec 11, 2003·Atherosclerosis. Supplements·John P Cooke
Jul 18, 2002·Clinical Genetics·C HeltianuM Simionescu
Mar 10, 2001·Clinical and Experimental Pharmacology & Physiology·A D Struthers
Jun 26, 2001·Seminars in Dialysis·B Descamps-LatschaV Witko-Sarsat
Apr 9, 2008·The Journal of Biological Chemistry·Ming-Xiang ZhangXing Li Wang
Dec 22, 2010·The Journal of Experimental Medicine·Patrizia NigroBradford C Berk
Apr 7, 2010·Metabolic Syndrome and Related Disorders·Manuel T Velasquez, James D Katz
Dec 17, 2011·European Heart Journal·Andreas J FlammerRoberto Corti
Sep 28, 1999·Journal of Cardiovascular Pharmacology·H Darius
Feb 15, 2000·Journal of Cardiovascular Pharmacology·W MaierT F Lüscher
Jan 29, 2002·Journal of Cardiovascular Pharmacology·T F LüscherF Cosentino
Jan 16, 2002·Journal of Cardiovascular Pharmacology·Navin Kumar ThakurAkihisa Iguchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.

Cardiology Journals

Discover the latest cardiology research in this collection of the top cardiology journals.