Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila

Open Biology
Barbara LaurinyeczRita Sinka

Abstract

Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correct lipid composition during sperm development and show that phosphatidic acid levels are crucial in late stages of spermatogenesis.

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Citations

Feb 11, 2018·Cellular and Molecular Life Sciences : CMLS·Ana L Santos, Giulio Preta
Jan 22, 2020·Journal of Evolutionary Biology·Ruijian Guo, Klaus Reinhardt
Mar 3, 2020·Frontiers in Cell and Developmental Biology·Nicholas J Blunsom, Shamshad Cockcroft
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May 4, 2020·Chemistry and Physics of Lipids·William Jennings, Richard M Epand
Jul 20, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·David D Lowe, Denise J Montell

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Methods Mentioned

BETA
PCR
transgenic
electron microscopy
Assay

Software Mentioned

ipid X plorer
iTEM
X calibur

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