Reduced expression of GNA11 and silencing of MCT1 in human breast cancers

Oncology
Kiyoshi AsadaToshikazu Ushijima

Abstract

Alteration in the methylation status of a gene is often associated with its altered expression. Based on a genome scanning technique for differences in CpG methylations, methylation-sensitive representational difference analysis, DNA fragments hypermethylated in a human breast cancer were isolated. A DNA fragment was isolated from intron 1 of guanine-nucleotide-binding protein alpha-11 (GNA11). mRNA expression of GNA11 was shown to be decreased in 10 of 16 breast cancers by RT-PCR analysis, and the immunoreactivity of the GNA11 product, Galpha11 subunit of heterotrimeric G-protein, was observed to be reduced in 14 of the 16 cancers by immunohistochemistry. Methylation of a CpG island (CGI) in the 5' region of GNA11 or that of intron 1 did not show a clear correlation with its decreased expression. Another DNA fragment was isolated from a CGI in the 5' upstream region of monocarboxylate transporter 1 (MCT1), and was methylated in 4 of 20 breast cancers. The CGI was also methylated in a human breast cancer cell line, MDA-MB-231, and quantitative RT-PCR showed that its expression was almost lost in the cell line. By treatment of the cells with a demethylating agent, 5-aza-2'-deoxycytidine, the methylation was removed and the expre...Continue Reading

Citations

Mar 13, 2012·Journal of Bioenergetics and Biomembranes·Céline PinheiroFátima Baltazar
Feb 19, 2005·Nature Reviews. Cancer·Toshikazu Ushijima
May 3, 2003·Genome Research·Toshikazu UshijimaKazuaki Miyamoto
Nov 27, 2008·The Journal of Clinical Investigation·Pierre SonveauxMark W Dewhirst
Sep 4, 2013·The Journal of Clinical Investigation·Joanne R Doherty, John L Cleveland
May 11, 2010·Journal of Biomedicine & Biotechnology·Céline PinheiroFátima Baltazar
Jul 25, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Céline PinheiroFátima Baltazar
Apr 9, 2005·International Journal of Cancer. Journal International Du Cancer·Kazuaki MiyamotoToshikazu Ushijima
May 2, 2003·Annals of the New York Academy of Sciences·Atsushi KanedaToshikazu Ushijima
Mar 20, 2016·Biochimica Et Biophysica Acta·Jhudit Pérez-EscuredoPierre Sonveaux
Jul 20, 2010·Histopathology·Céline PinheiroFátima Baltazar
Aug 18, 2004·Biochemical Pharmacology·Moshe SzyfShafaat A Rabbani
Mar 7, 2006·Biochemical and Biophysical Research Communications·Keon Wook KangHyo-Kyung Han
Apr 29, 2010·Cancer Biology & Therapy·L Isabel Cárdenas-NaviaYardena Samuels
Jan 12, 2017·Veterinary and Comparative Oncology·J G RushtonB Nell
Nov 1, 2013·Endocrine-related Cancer·Filipa Morais-SantosCéline Pinheiro
Jun 29, 2016·Clinical Pharmacology and Therapeutics·R S Jones, M E Morris
Nov 21, 2019·Current Molecular Medicine·Wenhui WangJinming Li
Mar 8, 2020·Pharmacological Reviews·Melanie A FelmleeMarilyn E Morris
Jul 24, 2015·Oncotarget·Filipa Morais-SantosCéline Pinheiro
Feb 1, 2006·Molecular Cancer Research : MCR·Jean SpenceDan Mercola
Jun 5, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Qiwei YangSusan L Cohn
Aug 10, 2019·Molecular Metabolism·Valéry L PayenPierre Sonveaux
May 29, 2021·Molecular Biology Reports·J R Dev ArundhathiChandra Prakash Prasad
Jun 9, 2021·Life Sciences·Vaishali ChandelDhruv Kumar

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