Reduced expression of Nrdp1 predicts a poor prognosis in human hepatocellular carcinoma

OncoTargets and Therapy
Xian ShaoZhong Chen

Abstract

Hepatocellular carcinoma (HCC) is an aggressive form of liver cancer with particularly poor survival rates for patients. Targeted molecular therapies are lacking, and current treatment is generally limited to surgical resection or liver transplantation. Overexpression and aberrant signaling of the ErbB family of receptors has been implicated in HCC, but the mechanisms underlying ErbB overexpression are unclear. In this study, we investigated the potential role of neuregulin receptor degradation protein-1 (Nrdp1), a regulator of ErbB3 protein stability, in HCC progression. We compared the expression of Nrdp1 in various HCC cell lines and in 8 pairs of tumor and peritumor tissue samples using Western blot analysis. Changes in the degree of proliferation were determined before and after small interfering RNA (siRNA)-induced knockdown of Nrdp1 using a cell counting Kit-8 (ccK-8) assay and cell-cycle analysis. The correlation between Nrdp1 expression and prognosis was determined in specimens of 89 HCC patients. Nrdp1 expression is significantly reduced in HCC tissues compared with adjacent healthy tissues. Higher Nrdp1 expression corresponds to lower maximal tumor size (χ2, P<0.05), lower histological grade (χ2, P<0.05), and higher ...Continue Reading

Citations

Jun 30, 2019·Expert Review of Molecular Diagnostics·Shinichi UmedaYasuhiro Kodera
Mar 20, 2019·Cancer Biotherapy & Radiopharmaceuticals·Qiangfeng YuJianyin Zhou
Jun 21, 2020·Cell Biochemistry and Biophysics·Yong LiuChengmin Xuan
Mar 10, 2021·Cancer Cell International·Yu ShuaiGuihai Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
transfection
protein assay
electrophoresis
flow cytometry
surgical resection

Software Mentioned

Cell Quest
Image Lab
Leica QWin Plus

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.