Reduced expression of purinergic P2X4 receptors increases voluntary ethanol intake in C57BL/6J mice

Alcohol
Sheraz KhojaDaryl L Davies

Abstract

Purinergic P2X4 receptors (P2X4Rs) belong to the P2X superfamily of ionotropic receptors that are gated by adenosine 5'-triphosphate (ATP). Accumulating evidence indicates that P2X4Rs play an important role in regulation of ethanol intake. At the molecular level, ethanol's inhibitory effects on P2X4Rs are antagonized by ivermectin (IVM), in part, via action on P2X4Rs. Behaviorally, male mice deficient in the p2rx4 gene (P2X4R knockout [KO]) have been shown to exhibit a transient increase in ethanol intake over a period of 4 days, as demonstrated by social and binge drinking paradigms. Furthermore, IVM reduced ethanol consumption in male and female rodents, whereas male P2X4R KO mice were less sensitive to the anti-alcohol effects of IVM, compared to wildtype (WT) mice, further supporting a role for P2X4Rs as targets of IVM's action. The current investigation extends testing the hypothesis that P2X4Rs play a role in regulation of ethanol intake. First, we tested the response of P2X4R KO mice to ethanol for a period of 5 weeks. Second, to gain insights into the changes in ethanol intake, we employed a lentivirus-shRNA (LV-shRNA) methodology to selectively knockdown P2X4R expression in the nucleus accumbens (NAc) core in male C57B...Continue Reading

References

Jan 1, 1993·Psychopharmacology·J K BelknapE R Young
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·C LiF F Weight
Aug 1, 1995·Progress in Neurobiology·M M Behbehani
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·F SotoW Stühmer
Mar 4, 1998·British Journal of Pharmacology·C LiF F Weight
Apr 9, 1999·Nature Neuroscience·Y H Jo, R Schlichter
Jul 16, 1999·Neurochemistry International·F F WeightR W Peoples
Jul 25, 2000·British Journal of Pharmacology·K XiongF F Weight
Mar 21, 2001·Nature Reviews. Neuroscience·B S Khakh
Mar 30, 2001·The Journal of Biological Chemistry·Q ShanJ W Lynch
Sep 25, 2002·Physiological Reviews·R Alan North
Nov 26, 2002·Behavioural Brain Research·Gaetano Di Chiara
Sep 17, 2004·Pharmacology & Therapeutics·Rueben A GonzalesWilliam M Doyon
Sep 1, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joan A SimFrancois Rassendren
Mar 25, 2008·Alcohol·Naomi YoneyamaDeborah A Finn
May 23, 2008·Neuroscience·Y PankratovA Verkhratsky
Jun 28, 2008·The Journal of Pharmacology and Experimental Therapeutics·Cheng XiaoJiang H Ye
Jul 2, 2008·Nature Reviews. Drug Discovery·Geoffrey Burnstock
Oct 31, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lauriane UlmannFrancois Rassendren
Oct 31, 2009·BMC Biology·Boris TabakoffUNKNOWN WHO/ISBRA Study on State and Trait Markers of Alcoholism
Dec 17, 2009·American Journal of Physiology. Endocrinology and Metabolism·Hana ZemkovaStanko S Stojilkovic
Jan 19, 2010·Biochimica Et Biophysica Acta·Eun Kyung Kim, Eui-Ju Choi
Jun 15, 2010·The Journal of Pharmacology and Experimental Therapeutics·Liana AsatryanDaryl L Davies
Jan 5, 2011·Neuropharmacology·Brian A McCool
Jan 8, 2011·The Journal of Pharmacology and Experimental Therapeutics·Olga OstrovskayaDaryl L Davies
Jan 13, 2011·Alcoholism, Clinical and Experimental Research·Liana AsatryanDoo-Sup Choi
Feb 18, 2011·The European Journal of Neuroscience·Ramón A LorcaBernardo Morales
Apr 13, 2011·The Journal of Biological Chemistry·Young-Hwan JoEric Boué-Grabot
Jul 14, 2011·The European Journal of Neuroscience·Andrew W BaxterR Alan North
Mar 31, 2012·Addiction Biology·Raye Z LittenAntonio Noronha
Apr 3, 2012·Neuropharmacology·Megan M YardleyDaryl L Davies
May 15, 2012·Pharmacology, Biochemistry, and Behavior·William J McBrideRichard L Bell
Apr 23, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Letisha R WyattDaryl L Davies
Nov 6, 2013·Frontiers in Cellular Neuroscience·Makoto TsudaKazuhide Inoue
Jan 24, 2014·The International Journal of Neuropsychopharmacology·Liana AsatryanDaryl L Davies
Mar 29, 2014·Neurochemical Research·Letisha R WyattDaryl L Davies
Aug 20, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Larisa GofmanRaghava Potula
Sep 4, 2015·Alcoholism, Clinical and Experimental Research·Kelle M FranklinWilliam J McBride

❮ Previous
Next ❯

Citations

Jul 14, 2020·Neuroreport·Larry RodriguezDaryl L Davies
Jun 2, 2020·Frontiers in Pharmacology·Leanne StokesElizabeth Allum
Aug 7, 2020·International Journal of Molecular Sciences·Alejandro MontillaMaria Domercq
Apr 4, 2021·International Journal of Molecular Sciences·Anastasia GeladarisMartin S Weber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Alcohol Use Disorder

Alcohol use disorder involves a pattern of alcohol consumption that includes compulsive use and a loss of control over intake of alcohol. The impact on physical health, socioeconomic factors, and psychiatric health is profound. Find the latest research on alcohol use disorder here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.

© 2022 Meta ULC. All rights reserved