Reduced expression of the transporter associated with antigen processing 1 molecule in malignant glioma cells, and its restoration by interferon-gamma and -beta

Journal of Neurosurgery
Eiji SatohHirofumi Naganuma

Abstract

It remains unclear whether malignant glioma cells can deliver tumor antigens efficiently to major histocompatibility complex (MHC) Class I molecules. To elucidate the mechanism of antigen presentation in malignant gliomas, the authors examined the expression of the transporter associated with antigen processing 1 (TAP1), which transports antigens to MHC Class I molecules, and low-molecular-mass polypeptide 2 (LMP2), which is a subunit of a proteasome. They also analyzed the effects of interferon (IFN)-gamma and IFN-beta on the expression of these molecules. Five glioma cells expressed undetectable or very low levels of TAP1 protein and did not express TAP1 messenger (m)RNA. Normal brain tissue and glioma tissue specimens also showed undetectable levels of TAP1 protein and the same levels of LMP2 protein as lymphoblastoid B cells. Treatments of the tumor cells with IFN-gamma, or -beta enhanced the expression of both TAP1 protein and mRNA as well as the expression of MHC Class I molecules. The expression of LMP2 protein was not altered by the IFN treatments. The authors analyzed structural alterations in the TAP1 promoter region in eight malignant glioma cell lines. Single nucleotide polymorphism was found in 446 bp up-stream of ...Continue Reading

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Citations

Feb 13, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jacky T YeungHideho Okada
Nov 5, 2011·Expert Review of Anticancer Therapy·Serena PellegattaGaetano Finocchiaro
Jul 7, 2015·Journal of Ophthalmology·Su ZhangNan Hu
Feb 10, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Eric M BlumanRichard M Terek
Jan 1, 2008·Gene Regulation and Systems Biology·Takuma HayashiIkuo Konishi
Jul 3, 2021·Viruses·Nicole Mihelson, Dorian B McGavern

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Methods Mentioned

BETA
flow cytometry
PCR

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