PMID: 11912288Mar 26, 2002Paper

Reduced expression of tissue inhibitor of metalloproteinase (TIMP)-2 in gestational trophoblastic diseases

Molecular Human Reproduction
Tomomitsu OkamotoMasami Osawa

Abstract

To elucidate the involvement of type IV collagenases [matrix metalloproteinase (MMP)-2 and MMP-9] and their tissue inhibitors (TIMP-1 and TIMP-2) in the development of gestational trophoblastic disease (GTD), we quantified their levels in hydatidiform mole and choriocarcinoma tissues using specific enzyme-linked immunosorbent assays, and the results were compared with those from normal first trimester placenta. Levels of pro-MMP-2 were increased in hydatidiform mole, and they were further elevated in choriocarcinoma. Levels of pro-MMP-9 in choriocarcinoma and those of TIMP-1 in both hydatidiform mole and choriocarcinoma were also increased. In contrast, TIMP-2 levels were markedly decreased in both hydatidiform mole and choriocarcinoma. Similar results were obtained by the tissue culture of first trimester placenta and hydatidiform mole. Gelatin zymography indicated that the levels of both pro- and activated forms of MMP-2 and MMP-9 were higher in hydatidiform mole and choriocarcinoma. The decreased expression of TIMP-2 in hydatidiform mole and choriocarcinoma was confirmed by Western blot, Northern blot and immunohistochemistry, with the decrease being more pronounced in choriocarcinoma. Taken together, the present study shows...Continue Reading

Citations

Oct 1, 2008·Indian Journal of Clinical Biochemistry : IJCB·Maya RocheK Nalini
Sep 14, 2004·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·J Q CuiJ Q Li
Sep 22, 2005·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·K K RasilaC Verschraegen
Aug 4, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·X WangJ J Kavanagh
Aug 4, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·P PetignatP Bischof
Dec 15, 2010·Future Oncology·Constantine Alifrangis, Michael J Seckl
Sep 1, 2009·Experimental and Molecular Pathology·Mahmoud ShokryMahmoud Rezk A Hussein
Mar 17, 2009·Journal of Clinical Laboratory Analysis·Martina MontagnanaGian Cesare Guidi
Feb 13, 2015·Molecular Cytogenetics·Jiadi WenEvica Rajcan-Separovic
Jan 14, 2012·Human & Experimental Toxicology·E González-PueblaB Sánchez-Ramírez

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