Reduced FOXM1 Expression Limits Trophoblast Migration and Angiogenesis and Is Associated With Preeclampsia

Reproductive Sciences
Lara J MonteiroSebastian E Illanes

Abstract

Trophoblast cells are often compared to highly invasive carcinoma cells due to their capacity to proliferate in hypoxic conditions and to exhibit analogous vascular, proliferative, migratory, and invasive capacities. Thus, genes that are important for tumorigenesis, such as forkhead box M1 ( FOXM1) may also be involved in processes of trophoblast invasion. Indeed, we found Foxm1 protein and messenger RNA (mRNA) levels decreased as gestational age increased in rat's placentae. Accordingly, when mimicking early placental events in vitro, protein and mRNA expression of FOXM1 increased from 21% to 8% O2, reaching its highest expression at 3% oxygen tension, which reflects early implantation environment, and dropping to very low levels at 1% O2. Remarkably, FOXM1 silencing in JEG-3 cells was able to significantly decrease migration by 27.9%, in comparison with those cells transfected with control siRNA. Moreover, angiogenesis was compromised when conditioned media (CM) from FOXM1-siRNA -JEG-3 (3% O2) was added to human umbilical vein endothelial cells (HUVEC) cells; however, when CM of JEG-3 cells overexpressing FOXM1 at 1% O2 was added, the ability of HUVEC to form tubule networks was restored. Additionally, quantitative real-time ...Continue Reading

References

Jan 1, 1997·American Journal of Reproductive Immunology : AJRI·D S Torry, R J Torry
Apr 21, 2001·Placenta·G J Burton, I Caniggia
Apr 9, 2005·The Journal of Biological Chemistry·Il-Man KimVladimir V Kalinichenko
Apr 21, 2005·The Journal of Clinical Endocrinology and Metabolism·Nima SoleymanlouIsabella Caniggia
Oct 20, 2005·Human Reproduction Update·J L JamesL W Chamley
Aug 29, 2006·World Journal of Gastroenterology : WJG·Peter S YooCharles H Cha
Oct 19, 2007·Nature Reviews. Cancer·Stephen S Myatt, Eric W-F Lam
Nov 3, 2007·BMJ : British Medical Journal·Leanne BellamyDavid J Williams
May 26, 2009·Seminars in Perinatology·Lelia Duley
Jul 3, 2009·Biology of Reproduction·Natalie J HannanLois A Salamonsen
Nov 21, 2009·Human Reproduction Update·K G PringleC T Roberts
Jul 6, 2010·Lancet·Eric A P SteegersRobert Pijnenborg
Feb 22, 2011·Best Practice & Research. Clinical Obstetrics & Gynaecology·Jennifer A HutcheonK S Joseph
Oct 8, 2011·Biochimica Et Biophysica Acta·Chuay-Yeng KooEric W-F Lam
Nov 6, 2012·Cold Spring Harbor Perspectives in Medicine·Ana Sofia Cerdeira, S Ananth Karumanchi
Jul 9, 2014·Nature Reviews. Nephrology·Tinnakorn ChaiworapongsaRoberto Romero
Jul 30, 2014·Seminars in Cancer Biology·Laura BellaEric W-F Lam
Oct 9, 2015·PloS One·Alejandra Perez-SepulvedaSebastián E Illanes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.