Reduced graphene oxide and gold nanoparticle composite-based solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of 9 mycotoxins in milk

Food Chemistry
Keqiu JiangZheng Han

Abstract

A reliable solid-phase extraction (SPE) procedure using reduced graphene oxide and gold nanoparticle (rGO/Au) composite as sorbent was proposed for purification and enrichment of aflatoxin B1 (AFB1), aflatoxin M1 (AFM1), ochratoxin A (OTA), zearalenone (ZEA), α-zearalenol (α-ZOL), β-zearalenol (β-ZOL), zearalanone (ZAN), α-zearalanol (α-ZAL) and β-zearalanol (β-ZAL) in milk. Main parameters affecting the performance of SPE procedure were thoroughly investigated. The optimized conditions included 2% acetonitrile in water as the loading solution, 5% methanol in water as the washing solution, and methanol/acetonitrile/formic acid (50/49/1, v/v/v) as the elution solvent. Satisfactory linearity (R2 ≥ 0.992), high sensitivity (limit of quantification in the range of 0.02-0.18 ng mL-1), adequate recovery (70.2-111.2%), and acceptable precision (RSD, 2.0-14.9%) were obtained when the optimal sample pretreatment protocol was combined with ultra-high-performance liquid chromatography-tandem mass spectrometry detection. The applicability of the validated method was further verified in real milk samples.

Citations

May 6, 2019·Electrophoresis·Siyao Liu, Michael Lämmerhofer
Feb 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natalia CasadoIsabel Sierra
Aug 4, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yizhi Yao, Miao Long
Jan 14, 2021·Toxins·Yun GuanKamil Kuca
Sep 10, 2019·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shiqian GaoZhanen Zhang

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