Reduced Graphene Oxide Modified the Interdigitated Chain Electrode for an Insulin Sensor

Sensors
Ajay Kumar YagatiSungbo Cho

Abstract

Insulin is a key regulator in glucose homeostasis and its deficiency or alternations in the human body causes various types of diabetic disorders. In this paper, we present the development of a reduced graphene oxide (rGO) modified interdigitated chain electrode (ICE) for direct capacitive detection of insulin. The impedance properties of rGO-ICE were characterized by equivalent circuit modeling. After an electrochemical deposition of rGO on ICE, the electrode was modified with self-assembled monolayers and insulin antibodies in order to achieve insulin binding reactions. The impedance spectra and capacitances were measured with respect to the concentrations of insulin and the capacitance change (ΔC) was analyzed to quantify insulin concentration. The antibody immobilized electrode showed an increment of ΔC according to the insulin concentration in human serum ranging from 1 ng/mL to 10 µg/mL. The proposed sensor is feasible for label-free and real-time measuring of the biomarker and for point-of-care diagnosis.

References

Jul 28, 2006·Biosensors & Bioelectronics·K Vengatajalabathy GobiNorio Miura
Jan 8, 2008·Electroanalysis·Jonathan S Daniels, Nader Pourmand
Sep 2, 2009·Analytica Chimica Acta·Ying Wang, Jinghong Li
Jun 21, 2011·Biosensors & Bioelectronics·Junhyoung AhnMin-Gon Kim
Dec 4, 2012·Biosensors & Bioelectronics·Jennifer Y GerasimovRebecca Y Lai
Aug 31, 2014·Analytica Chimica Acta·Ha-Wook JungJae-Chul Pyun

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Citations

Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Dec 12, 2018·ACS Sensors·Rebecca SoffeJames Geoffrey Chase

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
biosensors
ICE
chip

Software Mentioned

ZView

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