Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: a transcriptomics analysis of adjuvanted vaccines

Vaccine
Sylvia S N ReemersLonneke Vervelde

Abstract

To gain more insight in underlying mechanisms correlating to protection against avian influenza virus (AIV) infection, we investigated correlates of protection after AIV H9N2 infection and studied the contribution of different adjuvants to a protective response at host transcriptional level. One-day-old chickens were immunised with inactivated H9N2 supplemented with w/o, Al(OH)(3), CpG or without adjuvant. Two weeks later, birds were homologously challenged and at 1-4 days post challenge (d.p.c.) trachea and lung were collected. Birds immunised with H9N2+w/o or H9N2+Al(OH)(3) were protected against challenge infection and had lower viral RNA expression, less immune related genes induced after challenge, a lower amplitude of change of gene expression and smaller cellular influxes compared to the higher and prolonged gene expression in unprotected birds. We show that a limited number of differentially expressed genes correlates with reduced immune activation and subsequently reduced immunopathology after challenge with AIV.

References

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Citations

Jun 21, 2011·Veterinary Immunology and Immunopathology·Eveline D de GeusLonneke Vervelde
Apr 2, 2013·Developmental and Comparative Immunology·Eveline D de Geus, Lonneke Vervelde
Mar 3, 2012·PloS One·Sylvia S N ReemersChristine A Jansen
Apr 3, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eveline D de GeusLonneke Vervelde
May 10, 2020·Virology Journal·Barbara Małgorzata KalenikAgnieszka Sirko
Jul 11, 2018·Developmental and Comparative Immunology·Barbara Małgorzata KalenikAgnieszka Sirko

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