Reduced levels of stromal sex hormone-binding globulin and androgen receptor dysfunction in the sperm storage region of the rat epididymis

Reproduction : the Official Journal of the Society for the Study of Fertility
Fabiane de SantiEstela Sasso-Cerri

Abstract

The cauda epididymidis is the major sperm storage region whose androgenic supply, essential for the sperm viability, is provided by the vasculature and is dependent upon testosterone diffusion through the stromal tissue to reach the epithelial cells. We have focused our efforts on examining the regulation of this important epididymal region by evaluating the impact of the androgen disrupter cimetidine on the epithelial-stromal androgenic microenvironment. Male rats received 100 mg/kg cimetidine (CMTG) or saline (CG) for 50 days, serum testosterone levels were measured and the epididymal cauda region was processed for light and transmission electron microscopy. In the proximal cauda region, the duct diameter was measured and birefringent collagen in the stroma was quantified. TUNEL-labeled epithelial cells were quantified, and androgen receptor (AR), karyopherin alpha (KPNA) and sex hormone-binding globulin (SHBG) levels were analyzed by immunofluorescence and Western blot. CMTG showed reduced duct diameter and high number of apoptotic epithelial cells. In the epithelium, the total AR concentration and the KPNA immunoreactivity were reduced, and a weak/absent AR nuclear immunofluorescence was observed in contrast to the enhanced...Continue Reading

References

Feb 1, 1979·Gut·R W Spence, L R Celestin
Feb 1, 1979·The Journal of Clinical Endocrinology and Metabolism·J W Funder, J E Mercer
Feb 1, 1978·The Journal of Physiology·P Y Wong, C H Yeung
Jan 1, 1990·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·G SinneckerR A DeLellis
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·D H Van ThielB Susen
Jun 1, 1982·British Journal of Clinical Pharmacology·C WangK K Yeung
Jan 1, 1995·Microscopy Research and Technique·B T Hinton, M A Palladino
Oct 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·S A KrupenkoB J Danzo
Nov 4, 2000·Biology of Reproduction·L R FrançaL D Russell
Dec 26, 2001·Current Opinion in Structural Biology·Y M Chook, G Blobel
Oct 2, 2002·Obstetrics and Gynecology Clinics of North America·Geoffrey L Hammond
Dec 19, 2003·Micron : the International Research and Review Journal for Microscopy·P S Cerri, E Sasso-Cerri
Aug 18, 2005·Journal of Periodontal Research·P S Cerri, E Katchburian
Sep 6, 2005·Journal of Cell Science·Pascal FarlaAdriaan B Houtsmuller
Jan 4, 2006·Molecular and Cellular Endocrinology·Sanjay KumarRakesh K Tyagi
May 16, 2006·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·T K Andreassen
Jan 15, 2008·Reproductive Biology and Endocrinology : RB&E·Margaret O'BrienTerry J Smith
Mar 6, 2008·Journal of Cell Science·Mark L CutressDavid E Neal
Jul 29, 2008·Journal of Cellular and Molecular Medicine·Norihisa OkadaMamoru Isemura
Apr 16, 2009·The Journal of Reproduction and Development·Thuy T B VoEui-Bae Jeung
Aug 25, 2009·Molecular and Cellular Endocrinology·William RosnerNicholas A Romas
Nov 26, 2009·The International Journal of Biochemistry & Cell Biology·Nigel C BennettGlenda C Gobe
Jul 24, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elizabeth M SnyderBarry T Hinton
Oct 22, 2010·Cells, Tissues, Organs·Flávia L BeltrameEstela Sasso-Cerri
Oct 26, 2010·Asian Journal of Andrology·Adam J KoppersMoira K O'Bryan
Feb 19, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Martina KubecovaVladimir Bobek
Mar 29, 2011·Journal of Andrology·Winnie W C ShumSylvie Breton
May 27, 2011·Biology of Reproduction·Geoffrey L Hammond
Jun 15, 2011·Experimental Cell Research·Yasunao KamikawaYoshihiro Yoneda
Jul 19, 2011·Journal of Andrology·Bernard Robaire, Mahsa Hamzeh
Aug 2, 2011·Analytical Biochemistry·Jihyeon LimRuth H Angeletti

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Jul 30, 2019·Frontiers in Endocrinology·Flávia Luciana BeltrameEstela Sasso-Cerri

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