Reduced mitochondrial and ascorbate-glutathione activity after artificial ageing in soybean seed

Journal of Plant Physiology
Xia XinXinxiong Lu

Abstract

The effect of artificial ageing on the relationship between mitochondrial activities and the antioxidant system was studied in soybean seeds (Glycine max L. cv. Zhongdou No. 27). Ageing seeds for 18d and 41d at 40°C reduced germination from 99% to 52% and 0%, respectively. In comparison to the control, malondialdehyde content and leachate conductivity in aged seeds increased and were associated with membrane damage. Transmission electron microscopy and Percoll density gradient centrifugation showed that aged seeds mainly contained poorly developed mitochondria in which respiration and marker enzymes activities were significantly reduced. Heavy mitochondria isolated from the interface of the 21% and 40% Percoll were analyzed. Mitochondrial antioxidant enzymes activities including superoxide dismutase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase were significantly reduced in aged seeds. A decrease in total ascorbic acid (ASC) and glutathione (GSH) content as well as the reduced/oxidized ratio of ASC and GSH in mitochondria with prolonged ageing showed that artificial ageing reduced ASC-GSH cycle activity. These results suggested an elevated reactive oxygen species (R...Continue Reading

References

Jul 17, 1998·Current Opinion in Biotechnology·N Smirnoff
Jun 29, 2000·Trends in Biochemical Sciences·T G Frey, C A Mannella
Feb 13, 2001·Plant Physiology·D C LoganC J Leaver
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Ian M Moller
Dec 18, 2001·Plant Physiology·A H MillarC J Leaver
Jul 1, 1997·The Plant Cell·J. D. Bewley
May 26, 2004·Cryobiology·Christina WaltersPhillip C Stanwood
Sep 1, 1991·Plant Physiology·N R Madamanchi, R G Alscher
Sep 2, 2008·Journal of Plant Physiology·Moêz SmiriEzzedine El Ferjani
Oct 18, 2008·Comptes rendus biologies·Christophe BaillyFrançoise Corbineau
Jun 13, 2009·Plant & Cell Physiology·Guangkun YinXinming Jing
Sep 15, 2012·Planta·Chris CarrieJames Whelan

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Citations

Feb 13, 2016·Frontiers in Plant Science·Łukasz WojtylaMałgorzata Garnczarska
Feb 6, 2015·Plant, Cell & Environment·Gábor Kocsy
Dec 3, 2014·The Plant Journal : for Cell and Molecular Biology·Yu WangXiaofeng Wang
Jun 19, 2019·Plants·Katarzyna KurekEwelina Ratajczak
Feb 7, 2020·Environmental Science and Pollution Research International·Maryam SalehchehLeila Zeidooni
Apr 5, 2018·International Journal of Molecular Sciences·Chunli MaoPeisheng Mao
Sep 17, 2021·Frontiers in Plant Science·Xiuling ChenGuangkun Yin
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad Javad KhodayarLeila Zeidooni

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