Reduced mRNA expression levels of MBD2 and MBD3 in gastric carcinogenesis

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Thaís Brilhante PontesMarília Arruda Cardoso Smith

Abstract

Aberrant methylation has been reported in several neoplasias, including gastric cancer. The methyl-CpG-binding domain (MBD) family proteins have been implicated in the chromatin remodeling process, leading to the modulation of gene expression. To evaluate the role of MBD2 and MBD3 in gastric carcinogenesis and the possible association with clinicopathological characteristics, we assessed the mRNA levels and promoter methylation patterns in gastric tissues. In this study, MBD2 and MBD3 mRNA levels were determined by RT-qPCR in 28 neoplastic and adjacent nonneoplastic and 27 gastritis and non-gastritis samples. The promoter methylation status was determined by bisulfite sequencing, and we found reduced MBD2 and MBD3 levels in the neoplastic samples compared with the other groups. Moreover, a strong correlation between the MBD2 and MBD3 expression levels was observed in each set of paired samples. Our data also showed that the neoplastic tissues exhibited higher MBD2 promoter methylation than the other groups. Interestingly, the non-gastritis group was the only one with positive methylation in the MBD3 promoter region. Furthermore, a weak correlation between gene expression and methylation was observed. Therefore, our data suggest...Continue Reading

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Citations

Oct 24, 2014·Biology·Vichithra R B LiyanageJames R Davie
Jul 25, 2015·Journal of Cellular Biochemistry·Carolina Oliveira GigekMarilia Arruda Cardoso Smith
May 1, 2015·Epigenomics·Qian DuSusan J Clark
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Jul 25, 2017·Experimental and Molecular Pathology·Carolina Oliveira GigekMarilia Arruda Cardoso Smith
Jul 18, 2019·Genetic Testing and Molecular Biomarkers·Mei-Rong LiangOu-Ping Huang
Dec 25, 2019·Signal Transduction and Targeted Therapy·Yuan ChengXiawei Wei
Oct 30, 2020·OncoTargets and Therapy·Liantao LiJunnian Zheng
Dec 17, 2020·Signal Transduction and Targeted Therapy·Yuan ChengXiawei Wei
Jul 5, 2019·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Danilo L MenaldoSuely V Sampaio

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