Reduced neutrophil apoptosis in diabetic mice during staphylococcal infection leads to prolonged Tnfα production and reduced neutrophil clearance

PloS One
Frank HansesJean C Lee

Abstract

Diabetes is a frequent underlying medical condition among individuals with Staphylococcus aureus infections, and diabetic patients often suffer from chronic inflammation and prolonged infections. Neutrophils are the most abundant inflammatory cells during the early stages of bacterial diseases, and previous studies have reported deficiencies in neutrophil function in diabetic hosts. We challenged age-matched hyperglycemic and normoglycemic NOD mice intraperitoneally with S. aureus and evaluated the fate of neutrophils recruited to the peritoneal cavity. Neutrophils were more abundant in the peritoneal fluids of infected diabetic mice by 48 h after bacterial inoculation, and they showed prolonged viability ex vivo compared to neutrophils from infected nondiabetic mice. These differences correlated with reduced apoptosis of neutrophils from diabetic mice and were dependent upon the presence of S. aureus and a functional neutrophil respiratory burst. Decreased apoptosis correlated with impaired clearance of neutrophils by macrophages both in vitro and in vivo and prolonged production of proinflammatory tumor necrosis factor alpha by neutrophils from diabetic mice. Our results suggest that defects in neutrophil apoptosis may contri...Continue Reading

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Methods Mentioned

BETA
flow cytometry
amputations
fluorescence microscopy
lavage
density gradient centrifugation

Related Concepts

Cell Survival
Macrophage
Neutrophil Band Cells
Staphylococcal Infections
Staphylococcus aureus
Tumor Necrosis Factor-alpha
Apoptosis, Intrinsic Pathway
Complications of Diabetes Mellitus
Peritoneal Fluid (Body Substance)
Bacterial Infections

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