Reduced nucleic acid methylation impairs meiotic maturation and developmental potency of pig oocytes

Theriogenology
Yan-Kui WangCai-Xia Yang

Abstract

Oocyte meiosis is a complex process coordinated by multiple endocrinal and molecular circuits. Recently, N6-methyladenosine (m6A) epigenetic modification on RNA is revealed to be important for meiotic maturation. However, the molecular mechanism of how m6A modification exerts its effect on oocyte maturation is largely unknown. Here, we showed that endogenous m6A writers (Mettl3 and Wtap) and eraser (Fto) elevated their transcript levels during meiotic maturation of pig oocytes. From germinal vesicle (GV) to metaphase II (MII) stages, global m6A level significantly increased, and existed mostly in ooplasm. Methyl donor (betaine, 16 mM) treatment of porcine cumulus-oocyte complexes (COCs) during in vitro maturation (IVM) significantly boosted nucleic acid m6A level within oocytes, but unchanged meiotic process and oocyte subsequent development. By contrast, methylation inhibitor (cycloleucine, 20 mM) reduced nucleic acid m6A level, and significantly decreased the germinal vesicle breakdown (GVBD) rate, the extrusion rate of the first polar body, and the cleavage and blastocyst rates of parthenotes. In addition, in cycloleucine-treated oocytes Wtap increased but Lin28 decreased their abundances significantly, along with the higher...Continue Reading

Citations

Aug 11, 2020·Zygote : the Biology of Gametes and Early Embryos·Ruizhe LiShengmei Chen
May 20, 2020·Therapeutic Advances in Chronic Disease·Kaiwei XuJunlu Wang
Mar 11, 2020·Frontiers in Genetics·Mengfen WangXiangdong Liu
Nov 23, 2020·Archives of Gynecology and Obstetrics·Xiaoyan SunXuehong Zhang
Jan 13, 2021·Cell & Bioscience·Jianzhong GuXinbing Sui
Apr 24, 2021·Zygote : the Biology of Gametes and Early Embryos·Tong YuYunhai Zhang
Apr 7, 2021·Molecular Human Reproduction·Fang FangChengliang Xiong
Jul 3, 2021·Animals : an Open Access Journal From MDPI·Jindong HaoXianfeng Yu

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