Reduced numbers of mucosal DR(int) macrophages and increased numbers of CD103(+) dendritic cells during anti-TNF-α treatment in patients with Crohn's disease

Scandinavian Journal of Gastroenterology
A DigeJ Agnholt

Abstract

Anti-TNF-α treatment constitutes a mainstay in the treatment of Crohn's disease (CD), but its mechanisms of action are not fully understood. We aimed to investigate the effects of adalimumab, a human monoclonal TNF-α antibody, on macrophage (MQ) and dendritic cell (DC) subsets in mucosal biopsies and peripheral blood. Intestinal biopsies and blood samples were obtained from 12 different CD patients both before and 4 weeks after the initiation of the induction of adalimumab treatment. Endoscopic disease activity was estimated by the Simple Endoscopic Score for Crohn's Disease. Biopsies were obtained from inflamed and non-inflamed areas. The numbers of lamina propria CD14 (+) DR(int) and CD14 (+) DR(hi) MQs, CD141(+), CD141(-) and CD103(+) DCs subsets, and circulating monocytes and DCs were analyzed using flow cytometry. At baseline, we observed higher numbers of DR(int) MQs and lower numbers of CD103(+) DCs in inflamed versus non-inflamed mucosa [843 vs. 391/10(5) lamina propria mononuclear cells (LPMCs) (p < 0.05) and 9 vs. 19 × 10(5) LPMCs (p = 0.01), respectively]. After four weeks of adalimumab treatment, the numbers of DR(int) MQs decreased [843 to 379/10(5) LPMCs (p = 0.03)], whereas the numbers of CD103(+) DCs increased [...Continue Reading

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Citations

Sep 24, 2016·Journal of Crohn's & Colitis·Carolijn SmidsEllen G van Lochem
Jul 13, 2017·Inflammatory Bowel Diseases·Hiroshi MatsunoTsunekazu Mizushima
May 11, 2018·Immunopharmacology and Immunotoxicology·Christian L HvasJørgen Agnholt
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Mar 26, 2020·International Immunopharmacology·Yuning JiaZhong Huang
May 8, 2021·Journal of Crohn's & Colitis·Katja A SelinEduardo J Villablanca

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