Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose.

Applied and Environmental Microbiology
Marie JeppssonMarie F Gorwa-Grauslund

Abstract

In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD(+). In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene, one of the isogenes of 6-phosphogluconate dehydrogenase, or by disruption of the ZWF1 gene, which encodes glucose 6-phosphate dehydrogenase. Decreasing the phosphoglucose isomerase activity by 90% also lowered the pentose phosphate pathway flux. These modifications all resulted in lower xylitol yield and higher ethanol yield than in the control strains. TMB3255, carrying a disruption of ZWF1, gave the highest ethanol yield (0.41 g g(-1)) and the lowest xylitol yield (0.05 g g(-1)) reported for a xylose-fermenting recombinant S. cerevisiae strain, but also an 84% lower xylose consumption rate. The low xylose fermentation rate is probably due to limited NADPH-mediated xylose reduction. Metabolic flux modeling of TMB3255 confirmed that the...Continue Reading

References

Sep 1, 1987·Applied and Environmental Microbiology·A V SarthyB D Hall
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
May 9, 1998·Applied and Environmental Microbiology·N W HoA P Brainard
May 10, 2000·Applied Microbiology and Biotechnology·A EliassonB Hahn-Hägerdal
Feb 1, 1953·Journal of Cellular Physiology·A A ANDREASEN, T J B STIER
Nov 1, 1990·Applied and Environmental Microbiology·K Skoog, B Hahn-Hägerdal

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Citations

Sep 16, 2006·Applied Microbiology and Biotechnology·Kaisa KarhumaaMarie-F Gorwa-Grauslund
Feb 13, 2007·Applied Microbiology and Biotechnology·Bärbel Hahn-HägerdalMarie F Gorwa-Grauslund
Jul 3, 2009·Applied Microbiology and Biotechnology·Akinori MatsushikaShigeki Sawayama
Oct 3, 2008·Journal of Industrial Microbiology & Biotechnology·Daniela B GurpilharesInês C Roberto
Aug 18, 2010·Journal of Industrial Microbiology & Biotechnology·Aloke K BeraMiroslav Sedlak
Oct 12, 2013·Journal of Industrial Microbiology & Biotechnology·Laura SalusjärviLaura Ruohonen
Nov 28, 2012·World Journal of Microbiology & Biotechnology·Changying Guo, Ning Jiang
Apr 19, 2008·Applied Biochemistry and Biotechnology·Kripa RaoSasidhar Varanasi
May 24, 2011·Applied Biochemistry and Biotechnology·Alan M Shupe, Shijie Liu
Nov 8, 2013·Applied Biochemistry and Biotechnology·Vijai SinghPawan Kumar Dhar
May 20, 2011·Biochemistry·Amy M Weeks, Michelle C Y Chang
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dana J WohlbachAudrey P Gasch
Jun 5, 2007·Applied and Environmental Microbiology·H Wouter WisselinkAntonius J A van Maris
Jun 15, 2010·Applied and Environmental Microbiology·Jeffrey G Gardner, David H Keating
Oct 5, 2010·Applied and Environmental Microbiology·David RunquistMaurizio Bettiga
Feb 20, 2007·Applied and Environmental Microbiology·Hideshi YanaseKenji Okamoto
Feb 5, 2008·Applied and Environmental Microbiology·César FonsecaIsabel Spencer-Martins
Oct 9, 2003·Applied and Environmental Microbiology·Ritva VerhoPeter Richard
Aug 7, 2003·Applied and Environmental Microbiology·Christophe RocaLisbeth Olsson
Nov 6, 2004·Applied and Environmental Microbiology·Yong-Su JinThomas W Jeffries
May 7, 2004·Applied and Environmental Microbiology·Marco SondereggerUwe Sauer
Sep 10, 2011·Microbial Cell Factories·Fumio MatsudaAkihiko Kondo
Oct 13, 2006·Antonie van Leeuwenhoek·Antonius J A van MarisJack T Pronk
Mar 26, 2013·Biotechnology Advances·Soo Rin KimJin-Ho Seo

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