Reduced PDCD4 Expression Promotes Cell Growth Through PI3K/Akt Signaling in Non-Small Cell Lung Cancer.

Oncology Research
Yan ZhenBin Wu

Abstract

It is largely recognized that PDCD4 is frequently lost in tumors of various origins, including lung cancer, and its loss contributes to tumor progression. However, its role and molecular mechanism remain largely unexplored in non-small cell lung cancer (NSCLC). In this study, downregulated PDCD4 mRNA expression was found in NSCLC tissues compared to their corresponding paracarcinoma tissues and distal paracarcinoma tissues. Induced expression of PDCD4 inhibited cell growth and proliferation and cell cycle transition in vitro. Conversely, knocking down PDCD4 expression promoted cell growth and proliferation. Mechanistically, PDCD4 inactivated PI3K/Akt signaling and its downstream cell cycle factors CCND1 and CDK4 to regulate cell growth in NSCLC. Additionally, PI3K-specific inhibitor Ly294002 suppressed the expression of pPI3K (Tyr458), pAkt (Ser473), CCND1, and CDK4 in PC9-shPDCD4 and A549-shPDCD4 cells. Furthermore, Akt-specific inhibitor MK2206 inhibited the expression of pAkt (Ser473), CCND1, and CDK4 in PC9-shPDCD4 and A549-shPDCD4 cells. Taken together, our study provides evidence that PDCD4 inhibits cell growth through PI3K/Akt signaling in NSCLC and may be a potential therapeutic target for NSCLC.

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Citations

Sep 24, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Gaiping WangCuifang Chang
Sep 27, 2018·Molecular Cancer Therapeutics·Joel D MaustJudith S Sebolt-Leopold
Nov 3, 2020·Cancer Biology & Therapy·Quantang PangHongyu Lu
Nov 21, 2019·Current Molecular Medicine·Wenhui WangJinming Li
Aug 2, 2018·International Journal of Oncology·Pin GuoLijuan Zhang

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Methods Mentioned

BETA
FACS
Transfection
electrophoresis

Software Mentioned

SPSS

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AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

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