Reduced perlecan expression and accumulation in human carotid atherosclerotic lesions

Atherosclerosis
Phan-Kiet TranUlf Hedin

Abstract

Heparan sulfate in the extracellular matrix of the artery wall has been proposed to possess anti-atherogenic properties by interfering with lipoprotein retention, suppression of inflammation, and inhibition of smooth muscle cell growth. Previously, the amount of heparan sulfate in atherosclerotic lesions from humans and animals has been shown to be reduced but the identity or identities of the heparan sulfate molecules being down regulated in this disease are not known. In this study, atherosclerotic lesions were retrieved from 44 patients undergoing surgery for symptomatic carotid stenosis. Normal iliac arteries from organ donors were used as controls. Analysis of the specimens by gene microarray showed a selective reduction in perlecan gene expression, whereas, expression of the other heparan sulfate proteoglycans in the artery wall, agrin and collagen XVIII, remained unchanged. Expression of the large chondroitin sulfate proteoglycan, versican, also remained unchanged. Real-time PCR confirmed the decrease in perlecan gene expression and the unchanged expression of versican. The findings were supported by immunohistochemical analysis demonstrating a reduced accumulation of both perlecan core protein and heparan sulfate in car...Continue Reading

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Citations

Jun 28, 2008·Reviews in Endocrine & Metabolic Disorders·Lisa R Tannock, Victoria L King
Jul 25, 2009·Cellular and Molecular Life Sciences : CMLS·Catherine Kirn-SafranDaniel D Carson
Sep 15, 2009·Glycobiology·Annika AsplundGöran Bondjers
Nov 10, 2012·Atherosclerosis·C OsterholmU Hedin
Jul 12, 2011·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·A RazuvaevU Hedin
May 7, 2014·Free Radical Research·C Y ChuangM J Davies
Jan 5, 2020·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Murat AkbasHabib Ozdemir
May 10, 2018·Frontiers in Physiology·Sandra Pérez-BaosGabriel Herrero-Beaumont
Jan 24, 2020·International Journal of Molecular Sciences·Amanda L TroutGregory J Bix
Mar 9, 2021·The Journal of Biological Chemistry·Chun-Yi NgMegan S Lord

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