Reduced plasma cholesterol and increased fecal sterol loss in multidrug resistance gene 2 P-glycoprotein-deficient mice

Gastroenterology
P J VosholF Kuipers

Abstract

mdr2 P-glycoprotein (Pgp) deficiency in mice leads to the absence of biliary phospholipids and cholesterol in the presence of normal bile salt secretion. The aim of this study was to evaluate the importance of the biliary pathway in cholesterol homeostasis by determining the effects of mdr2 Pgp deficiency on hepatic and plasma lipid levels and cholesterol kinetics in chow-fed mice. Hepatic lipid content, enzyme activities, plasma lipoprotein levels, and fecal sterol excretion were measured in wild-type (+/+) and mdr2 Pgp-deficient (-/-) mice. Cholesterol kinetics were determined using radiotracer techniques. No differences in hepatic lipid content were observed between (-/-) and (+/+) mice. Plasma high-density lipoprotein cholesterol and apolipoprotein A-I levels were strongly reduced in (-/-) mice compared with controls, whereas the apolipoprotein B contents of very-low-density lipoprotein and low-density lipoprotein were increased. Hepatic activity of 3-hydroxy-3-methylglutaryl-coenzyme A reductase was threefold greater in (-/-) mice than in controls; however, compartmental analysis of plasma cholesterol decay showed no differences in cholesterol synthesis between (-/-) and (+/+) mice. A dual isotope approach for estimating c...Continue Reading

Citations

Mar 17, 2004·Toxicology Letters·Marie-Yvonne AkoumeIbrahim M Yousef
Apr 3, 2001·Journal of Hepatology·M Fuchs, E F Stange
Jan 21, 2012·Current Opinion in Lipidology·Ryan E Temel, J Mark Brown
Jan 28, 2010·Pharmacological Reviews·Curtis D Klaassen, Lauren M Aleksunes
Sep 28, 2006·Annual Review of Physiology·David Q-H Wang
Sep 19, 2001·The Journal of Clinical Investigation·A K GroenF Kuipers
Nov 5, 1998·The Journal of Clinical Investigation·R P ElferinkA K Groen
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Torsten PlöschFolkert Kuipers
Sep 30, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mei ZhouLei Ling
May 25, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Christian V HulzebosFolkert Kuipers
Sep 12, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Bruno Stieger
Apr 16, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Anna BaghdasaryanMichael Trauner
Feb 21, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tamara PotikhaDaniel Goldenberg
Oct 30, 2012·Biochimica Et Biophysica Acta·Jonathan A ColemanRobert S Molday
May 2, 2015·Trends in Pharmacological Sciences·Ryan E Temel, J Mark Brown
Mar 30, 2010·Journal of Hepatology·Milka SokolovićAlbert K Groen
Nov 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C D JolleyS D Turley
Jul 10, 2009·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S H LeeC-K Shim
Dec 18, 2004·Journal of Lipid Research·Jelske N van der VeenFolkert Kuipers
Jun 18, 2016·Journal of Lipid Research·Marleen SchonewilleAlbert K Groen
Nov 8, 2014·Journal of Lipid Research·Jin WangHelen H Hobbs
Jul 30, 2014·Journal of Lipid Research·Chiara DegirolamoAntonio Moschetta
Sep 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Xiaosong Wang, Beverly Paigen
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Robert J AielloOmar L Francone
Apr 15, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Torsten PlöschFolkert Kuipers
Sep 12, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anniek WernerHenkjan J Verkade
May 16, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·David Q-H WangMartin C Carey
Jun 30, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karin E R GooijertHenkjan J Verkade
Jul 10, 2002·The Journal of Biological Chemistry·Torsten PlōschFolkert Kuipers

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