Reduced plasma RNA integrity in nasopharyngeal carcinoma patients

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Blenda C K WongY M Dennis Lo

Abstract

Recent research has shown the feasibility of detecting cell-free RNA markers in human subjects. As elevated RNase activity has previously been described in the circulation of cancer patients, we hypothesized that cancer patients may have reduced plasma RNA integrity. In this study, we used nasopharyngeal carcinoma (NPC) as a model system to test this hypothesis. Plasma RNA integrity was determined using the ratio of the concentrations of transcript sequences corresponding to the 3' to those from the 5' end of a housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Transcript concentrations were measured using real-time quantitative reverse transcription-PCR assays targeting the 5' and 3' regions. We analyzed the plasma RNA integrity in 49 untreated NPC patients and 53 healthy controls. We also assessed the plasma samples from 19 NPC patients before and after radiotherapy to further show the clinical potential of this marker. The 3' to 5' GAPDH ratio was significantly lower in the plasma of untreated NPC patients when compared with healthy controls (0.0252 versus 0.0485, P = 0.024). Statistical analysis showed that plasma GAPDH ratio was correlated with tumor stage but not with sex and age. Moreover, 14 of 19 NPC ...Continue Reading

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Citations

May 13, 2011·Nature Reviews. Cancer·Heidi SchwarzenbachKlaus Pantel
Jan 4, 2007·Molecular Cancer·William Chi-Shing Cho
Aug 13, 2015·Intensive Care Medicine Experimental·John H BoydKeith R Walley
Feb 27, 2010·Cellular & Molecular Biology Letters·Marketa UrbanovaJan Betka
Sep 15, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lanny GovMelissa B Lodoen
Aug 29, 2019·International Journal of Cancer. Journal International Du Cancer·Jian YuWei-Ping Zhou
Jan 4, 2018·The Journal of Biological Chemistry·Joshua H CookMelissa B Lodoen
Jul 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·K C Allen ChanY M Dennis Lo

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