Reduced pulmonary artery vasoconstriction in methacholine in cholesterol-fed rabbits

Hypertension
S L Pfister, W B Campbell

Abstract

Alterations in vascular tone are well documented in hypercholesterolemia, yet little is known about the role of dietary cholesterol in endothelium-dependent contractions of pulmonary arteries. Methacholine and arachidonic acid cause endothelium-dependent contractions in normal rabbit pulmonary artery that are mediated by thromboxane A2. We tested the effect of these agonists on pulmonary arteries from rabbits fed standard rabbit chow or chow supplemented with 2% cholesterol for 2 weeks. Arachidonic acid-induced contractions did not differ in the groups. However, methacholine-induced contractions were significantly depressed in cholesterol-fed rabbits. Vascular thromboxane A2 production was similar in normal and cholesterol-fed rabbits. Pretreatment with the nitric oxide synthase inhibitor nitro-L-arginine had no effect on contractions observed with methacholine in normal rabbits but enhanced methacholine-induced contractions in cholesterol-fed rabbits. In norepinephrine-precontracted vessels, methacholine caused a small relaxation response in normal rabbits. In contrast, in cholesterol-fed rabbits, methacholine produced enhanced relaxations, suggesting that cholesterol feeding augments relaxations and decreases contractions by ...Continue Reading

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Citations

Feb 16, 2017·Scientific Reports·Grzegorz KopećPiotr Podolec
May 10, 2017·Cardiovascular Research·Oana SoropDaphne Merkus
Dec 7, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Xin TangWilliam B Campbell
Oct 26, 2013·Journal of Applied Physiology·Vincent J de BeerM Harold Laughlin

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