Aug 8, 2018

Reduced-representation sequencing identifies small effective population sizes of Anopheles gambiae in the north-western Lake Victoria basin, Uganda

Malaria Journal
Rachel M WiltshireF H Collins

Abstract

Malaria is the leading cause of global paediatric mortality in children below 5 years of age. The number of fatalities has reduced significantly due to an expansion of control interventions but the development of new technologies remains necessary in order to achieve elimination. Recent attention has been focused on the release of genetically modified (GM) mosquitoes into natural vector populations as a mechanism of interrupting parasite transmission but despite successful in vivo laboratory studies, a detailed population genetic assessment, which must first precede any proposed field trial, has yet to be undertaken systematically. Here, the genetic structure of Anopheles gambiae populations in north-western Lake Victoria is explored to assess their suitability as candidates for a pilot field study release of GM mosquitoes. 478 Anopheles gambiae mosquitoes were collected from six locations and a subset (N = 96) was selected for restriction site-associated DNA sequencing (RADseq). The resulting single nucleotide polymorphism (SNP) marker set was analysed for effective size (Ne), connectivity and population structure (PCA, FST). 5175 high-quality genome-wide SNPs were identified. A principal components analysis (PCA) of the colli...Continue Reading

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Mentioned in this Paper

Genetic Drift
Biological Markers
Genome-Wide Association Study
Study
In Vivo
Size
Laboratory Studies
Genome
Genes
Genetic Analysis

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