Reduced satellite cell number in situ in muscular contractures from children with cerebral palsy

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Sudarshan DayanidhiRichard L Lieber

Abstract

Satellite cells (SC) are quiescent adult muscle stem cells critical for postnatal development. Children with cerebral palsy have impaired muscular growth and develop contractures. While flow cytometry previously demonstrated a reduced SC population, extracellular matrix abnormalities may influence the cell isolation methods used, systematically isolating fewer cells from CP muscle and creating a biased result. Consequently, the purpose of this study was to use immunohistochemistry on serial muscle sections to quantify SC in situ. Serial cross-sections from human gracilis muscle biopsies (n = 11) were labeled with fluorescent antibodies for Pax7 (SC transcriptional marker), laminin (basal lamina), and 4',6-diamidino-2-phenylindole (nuclei). Fluorescence microscopy under high magnification was used to identify SC based on labeling and location. Mean SC/100 myofibers was reduced by ∼70% (p < 0.001) in children with CP (2.89 ± 0.39) compared to TD children (8.77 ± 0.79). Furthermore, SC distribution across fields was different (p < 0.05) with increased percentage of SC in fields being solitary cells (p < 0.01) in children with CP. Quantification of SC number in situ, without any other tissue manipulation confirms children with spas...Continue Reading

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Citations

Apr 23, 2016·Developmental Medicine and Child Neurology·Bernard Dan
Aug 16, 2016·Muscle & Nerve·Andrew M ZogbyRichard L Lieber
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Feb 29, 2020·Muscle & Nerve·Glen H NuckollsRichard L Lieber
Dec 21, 2018·Journal of Applied Physiology·Richard L Lieber, Jan Fridén
Jan 12, 2021·Developmental Medicine and Child Neurology·Richard L Lieber, Tim Theologis
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Jun 7, 2021·Developmental Medicine and Child Neurology·Mariz Vainzof, Juliana Gurgel-Giannetti
Jul 26, 2021·Journal of Biomechanics·Peter B DykstraRichard L Lieber
Sep 10, 2021·Developmental Medicine and Child Neurology·Jason J HowardAdam P Shortland
Sep 25, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lydia A SibleyAndrea A Domenighetti

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