Reduced stem cell factor links smooth myopathy and loss of interstitial cells of cajal in murine diabetic gastroparesis

Gastroenterology
Viktor J HorváthTamás Ordög

Abstract

Diabetic gastroparesis involves neuropathy, myopathy, and depletion of interstitial cells of Cajal (ICC), which may cause dysrhythmias and impaired neural control. Most murine gastric ICC depend on stem cell factor (SCF) signaling but can also be maintained with insulin or insulin-like growth factor-I (IGF-I). We investigated whether SCF could mediate the actions of insulin and IGF-I. Expression of insulin receptor, IGF-I receptor, and SCF was studied in gastric muscles and purified ICC by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). The effects of insulin/IGF-I deficiency on SCF, ICC, smooth muscle, and neurons were investigated in nonobese diabetic mice and organotypic cultures by immunohistochemistry, microarrays, and/or quantitative RT-PCR. ICC in organotypic cultures were also studied after immunoneutralization of endogenous SCF. Insulin and IGF-I receptors were detected in smooth-muscle cells and myenteric neurons but not in ICC. Cell-surface expression of SCF was only found in smooth-muscle cells. ICC depletion in diabetes was accompanied by smooth-muscle atrophy and reduced SCF, whereas neuron-specific gene expression remained unchanged. In organotypic cultures, prevention of ICC lo...Continue Reading

Associated Clinical Trials

Citations

Feb 9, 2011·Gastroenterology·Madhusudan GroverUNKNOWN NIDDK Gastroparesis Clinical Research Consortium
Jun 18, 2009·The Journal of Biological Chemistry·Mira M WoutersGianrico Farrugia
Jun 28, 2013·Journal of the Royal Society, Interface·Jerry GaoLeo K Cheng
Mar 18, 2008·Journal of Gastroenterology and Hepatology·Takahiro YamamotoNorio Hayashi
Apr 10, 2008·Journal of Gastroenterology and Hepatology·Bridget R Southwell
Apr 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pedro J Gomez-PinillaGianrico Farrugia
Oct 8, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ketrija TouwB Paul Herring
Nov 22, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jie ChenJiande D Z Chen
Jun 8, 2013·World Journal of Gastroenterology : WJG·Yun WangLin Lin
Jun 27, 2013·World Journal of Gastroenterology : WJG·Jie ChenWei Cai
Jan 26, 2013·Journal of Neurogastroenterology and Motility·Jung Hwan Oh, Pankaj J Pasricha
May 2, 2014·World Journal of Gastroenterology : WJG·Hong-Li LuWen-Xie Xu
Jul 21, 2015·Expert Opinion on Pharmacotherapy·Mark MalamoodRon Schey
Mar 29, 2008·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C H Knowles, R De Giorgio
Dec 12, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·S M MillerG Farrugia
Nov 1, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B Chandrasekharan, S Srinivasan
Aug 30, 2007·Diabetic Medicine : a Journal of the British Diabetic Association·J FarajB Ohlsson
Sep 23, 2006·Journal of Cellular and Molecular Medicine·Jing YeJan D Huizinga
Dec 30, 2014·Journal of Neurogastroenterology and Motility·Jae Hyung ParkKyung Sik Park
Feb 4, 2016·Expert Review of Gastroenterology & Hepatology·James LangworthyRon Schey
Jan 28, 2017·Expert Opinion on Investigational Drugs·Gareth J Sanger, Pankaj Jay Pasricha
Oct 6, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J-W LiY Xu
Jan 10, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·M GroverUNKNOWN NIDDK Gastroparesis Clinical Research Consortium (GpCRC)
Apr 24, 2015·JPEN. Journal of Parenteral and Enteral Nutrition·Jie ZhuHaiyan Huang
Sep 22, 2007·Current Gastroenterology Reports·William L Hasler
Dec 17, 2008·Expert Review of Gastroenterology & Hepatology·William L Hasler
Apr 9, 2008·Gastroenterology·Jan D Huizinga, Elizabeth J White
Sep 2, 2006·The Journal of Physiology·Kenton M Sanders
Jul 2, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·X-Y WangL W C Liu
Jul 7, 2009·Biosensors & Bioelectronics·Shinsuke NakayamaKenzo Hirose
Feb 11, 2015·Gastroenterology Clinics of North America·Pankaj Jay Pasricha
Dec 18, 2016·Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology·V G M Prasad, Philip Abraham
Oct 21, 2017·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·B P HerringJ M Wo
Feb 7, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Feng JiangYu-Gen Chen
Sep 7, 2013·Pediatric Gastroenterology, Hepatology & Nutrition·Sun Hwan Bae
Aug 28, 2018·Journal of Cellular Physiology·Meng-Juan LinBao-Ping Yu
Jun 2, 2007·Nature Clinical Practice. Gastroenterology & Hepatology·Harsha VittalPankaj J Pasricha
Nov 23, 2006·Current Diabetes Reports·Liza K PhillipsMichael Horowitz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.